Rufato Fernanda Cristina Toloi, de Sousa Luiz Gustavo, Scalize Priscilla Hakime, Gimenes Rossano, Regalo Isabela Hallak, Rosa Adalberto Luiz, Beloti Marcio Mateus, de Oliveira Fabíola Singaretti, Bombonato-Prado Karina Fittipaldi, Regalo Simone Cecilio Hallak, Siéssere Selma
Department of Basic and Oral Biology, School of Dentistry of Ribeirão Preto, University of São Paulo, Avenida Do Café S/N, Ribeirão Preto, SP, 14040-904, Brazil.
Institute of Physics and Chemistry, Federal University of Itajubá, Itajubá, MG, Brazil.
Clin Oral Investig. 2022 Jan;26(1):1053-1065. doi: 10.1007/s00784-021-04089-y. Epub 2021 Aug 9.
The purpose of this investigation was to evaluate in vivo the response of bone tissue to photobiomodulation when associated with texturized P(VDF-TrFE)/BT in calvaria defects of ovariectomized rats.
Wistar Hannover rats were submitted to ovariectomy/control surgery. Calvaria bone defects of 5-mm diameter were performed after 90 days of ovariectomy. The animals were divided into OVX (without laser (L) and membrane), OVX + P(VDF-TrFE)/BT, OVX + P(VDF-TrFE)/BT + L, and OVX + PTFE + L. It was utilized a low-intensity gallium-aluminum-arsenide laser (GaAlAs) with 780-nm wavelength and 30-J/cm energy density in 12 sessions (120 s). Thirty days after the bone defect the animals were euthanized for histological, microtomographic, and molecular evaluation. Quantitative analysis was analyzed by statistical software for p < 0.05.
Histological parameters showed bone tissue formation at the borders of all group defects. The association of photobiomodulation and texturized P(VDF-TrFE)/BT was not synergistic and did not show significant changes in morphometric analysis and biomarkers gene expression. Nevertheless, texturized P(VDF-TrFE)/BT membrane enhanced bone repair regardless of the association with photobiomodulation therapy, with an increase of connectivity density when compared to the OVX + PTFE + L group. The association of photobiomodulation therapy and PTFE was synergistic, increasing the expression of Runx2, Alp, Bsp, Bglap, Sp7, and Rankl, even though not enough to reflect significance in the morphometric parameters.
The utilization of texturized P (VDF-TrFE)/BT, regardless of the association with photobiomodulation therapy, enhanced bone repair in an experimental model of osteoporosis.
本研究旨在评估去卵巢大鼠颅骨缺损处骨组织在与纹理化聚偏氟乙烯-三氟乙烯共聚物/钛酸钡（P(VDF-TrFE)/BT）联合应用时对光生物调节的体内反应。
将Wistar Hannover大鼠进行去卵巢/对照手术。去卵巢90天后制作直径5毫米的颅骨骨缺损。动物分为去卵巢组（无激光（L）和膜）、去卵巢+P(VDF-TrFE)/BT组、去卵巢+P(VDF-TrFE)/BT+L组和去卵巢+聚四氟乙烯（PTFE）+L组。使用波长780纳米、能量密度30焦/平方厘米的低强度砷化镓铝激光（GaAlAs），分12次照射（每次120秒）。骨缺损30天后，对动物实施安乐死以进行组织学、显微断层扫描和分子评估。通过统计软件对p<0.05进行定量分析。
组织学参数显示所有组缺损边缘均有骨组织形成。光生物调节与纹理化P(VDF-TrFE)/BT联合应用无协同作用，形态计量分析和生物标志物基因表达未显示显著变化。然而，无论是否与光生物调节治疗联合，纹理化P(VDF-TrFE)/BT膜均能促进骨修复，与去卵巢+PTFE+L组相比，连接密度增加。光生物调节治疗与PTFE联合应用具有协同作用，增加了Runx2、Alp、Bsp、Bglap、Sp7和Rankl的表达，尽管不足以在形态计量参数上体现出显著性。
在骨质疏松实验模型中，无论是否与光生物调节治疗联合，纹理化P(VDF-TrFE)/BT的应用均能促进骨修复。
