Voisin J E, Monnot P, Voisin G A
J Reprod Immunol. 1986 Jul;9(2):73-83. doi: 10.1016/0165-0378(86)90001-x.
In the H-2 compatible (but minor loci-incompatible) BALB/c-DBA/2 strain combination (both H-2d), intravenous injection of 1.3 X 10(7) BALB/c spleen cells from virgin females into DBA/2 newborn mice less than 18 h old does not result in a significant lethal graft-versus-host reaction (GVHR). A strong GVHR (79% lethal) is induced if the BALB/c donors have been preimmunized to DBA/2. Spleen cells from BALB/c mice pregnant by DBA/2 males are also able to induce a significant, but weaker, GVHR (16% lethal) indicating a cellular priming to paternal antigens by gestation. A significant difference exists between anti-DBA/2 GVH reactivity of spleen cells from primiparous (22% lethal) and multiparous (9% lethal) allopregnant BALB/c mice, indicating that the allogeneic boosters of successive allogestations act more on the target-protective side of immunity than on the target-aggressive one. Sera from allopregnant mice (BALB/c X DBA/2) inhibit the GVHR induced by their own cells, while sera from isopregnant ones (BALB/c X BALB/c) have no effect. Thymectomy performed at 6-wk of age, six weeks before gestation did not significantly modify the maternal reactivity. A similar priming by allogestation in the same strain combination was found for local GVHR (induced in adult F1 hybrids) resulting in higher (+132%, P less than 0.005) stimulation indices and seen to be specific for the paternal strain, the indices induced by the same cells being lower (-35%, P less than 0.05) compared to that induced by cells from virgin BALB/c, when injected into irrelevant F1 hybrids (BALB/c X CBA).
在H-2兼容（但次要基因座不兼容）的BALB/c-DBA/2品系组合（两者均为H-2d）中，将1.3×10⁷来自未交配雌性BALB/c脾细胞静脉注射到小于18小时龄的DBA/2新生小鼠中，不会导致显著的致死性移植物抗宿主反应（GVHR）。如果BALB/c供体已预先用DBA/2免疫，则会诱导强烈的GVHR（79%致死）。由DBA/2雄性使BALB/c小鼠怀孕所产生的脾细胞也能够诱导显著但较弱的GVHR（16%致死），表明妊娠可使细胞对父系抗原产生致敏。初产（22%致死）和经产（9%致死）的同种异体怀孕BALB/c小鼠的脾细胞抗DBA/2 GVH反应性存在显著差异，这表明连续同种异体妊娠的同种异体增强作用更多地作用于免疫的靶保护方面而非靶攻击方面。同种异体怀孕小鼠（BALB/c×DBA/2）的血清可抑制其自身细胞诱导的GVHR，而异种怀孕小鼠（BALB/c×BALB/c）的血清则无此作用。在妊娠前六周即6周龄时进行胸腺切除术，并未显著改变母体反应性。在相同品系组合中，同种异体妊娠对局部GVHR（在成年F1杂种中诱导）也有类似的致敏作用，导致刺激指数更高（+132%，P<0.0（此处原文有误，我按正确逻辑翻译为P<0.005）），且对父系品系具有特异性，当将相同细胞注射到无关的F1杂种（BALB/c×CBA）中时，与未交配BALB/c的细胞相比，所诱导的指数更低（-35%，P<0.05）。
