Shandong Provincial Key Laboratory of Biochemical Engineering, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China.
Shandong Provincial Key Laboratory of Biochemical Engineering, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China.
Fish Shellfish Immunol. 2023 Sep;140:108957. doi: 10.1016/j.fsi.2023.108957. Epub 2023 Jul 17.
Previous studies have shown that GIGYF2 plays multiple roles, but its overall biological function remains poor-defined. Here we clearly demonstrated that zebrafish (Danio rerio) GIGYF2 has GYF domain and gigyf2 mainly expressed in caudal fin, brain, eyes and testis in a tissue specific manner, and was most abundant in brain and testis. GYF domain of GIGYF2 was a peptidoglycan (PGN), lipopolysaccharide (LPS)- and lipoteichoic acid (LTA)- binding protein abundantly stored in the testis/embryos of zebrafish, acting not only as a pattern recognition receptor, but also as an effector molecule, capable of inhibiting the growth of gram-positive and -negative bacteria. Furthermore, we reveal that the residues of GIGYF2 positioned at 582-601 and 848-865 were indispensable for GIGYF2 antibacterial activity. Additionally, site-directed mutation could improve antibacterial activities. Collectively, our results indicate that zebrafish GYF domain of GIGYF2 recognize bacterial characteristic molecules PGN, LPS and LTA, and directly kill bacteria as an antibacterial effector. This work also provides another angle for understanding the biological roles of GIGYF2.
先前的研究表明 GIGYF2 发挥着多种作用，但它的整体生物学功能仍未得到明确界定。在这里，我们明确地表明，斑马鱼（Danio rerio）GIGYF2 具有 GYF 结构域，并且 gigyf2 主要以组织特异性的方式在尾鳍、大脑、眼睛和睾丸中表达，在大脑和睾丸中最为丰富。GIGYF2 的 GYF 结构域是一种肽聚糖（PGN）、脂多糖（LPS）和脂磷壁酸（LTA）结合蛋白，在斑马鱼的睾丸/胚胎中大量储存，不仅作为模式识别受体，而且作为效应分子，能够抑制革兰氏阳性和革兰氏阴性细菌的生长。此外，我们揭示了 GIGYF2 位于 582-601 和 848-865 位的残基对于 GIGYF2 的抗菌活性是不可或缺的。此外，定点突变可以提高抗菌活性。总的来说，我们的结果表明，斑马鱼 GIGYF2 的 GYF 结构域识别细菌特征分子 PGN、LPS 和 LTA，并直接作为抗菌效应物杀死细菌。这项工作也为理解 GIGYF2 的生物学作用提供了另一个角度。
