Zivin J A, Waud D R
J Pharmacol Methods. 1986 Aug;16(1):1-22. doi: 10.1016/0160-5402(86)90025-2.
Statistical methods for analyzing enzyme kinetic or drug binding assays involving simple first-order kinetics have been reviewed. The Eadie-Hofstee format combines many attractive features. Specifically, this plot is as efficient as any of the alternatives, shows no tendency to produce grossly aberrant values, does not require explicit weighting, does not require an iterative fitting process, gives the parameters of interest and their errors of estimate directly, and is unbiased (after correction). A correction for the bias inherent in the Eadie-Hofstee format has been developed. A numerical example and computer program are given in appendices.
已对用于分析涉及简单一级动力学的酶动力学或药物结合试验的统计方法进行了综述。伊迪-霍夫斯泰格式具有许多吸引人的特点。具体而言，这种作图法与其他任何方法一样有效，不会产生严重异常值，不需要明确加权，不需要迭代拟合过程，能直接给出感兴趣的参数及其估计误差，并且（经过校正后）无偏差。已针对伊迪-霍夫斯泰格式中固有的偏差开发了一种校正方法。附录中给出了一个数值示例和计算机程序。
