协同性以及绘制结合和稳态动力学数据的方法。
Co-operativity and the methods of plotting binding and steady-state kinetic data.
作者信息
Whitehead E P
出版信息
Biochem J. 1978 May 1;171(2):501-4. doi: 10.1042/bj1710501.
Abstract
The features that distinguish positive from negative co-operativity in double-reciprocal Eadie-Hofstee-Scatchard and Hanes plots, often incorrectly stated to be the sign of curvature or second derivatives, are explained. It is shown how to determine the 'Hill exponent' and interaciton free energies from curves in these plots, and in the simple plot of ligand binding or velocity against free ligand or substrate concentration. New types of plots, where the kind of co-operative behaviour is more obvious than in the traditional ones, are proposed.
摘要
解释了双倒数伊迪-霍夫斯泰-斯卡查德图和黑尼斯图中区分正协同性和负协同性的特征，这些特征常被错误地表述为曲率或二阶导数的符号。展示了如何从这些图中的曲线以及配体结合或速度对游离配体或底物浓度的简单图中确定“希尔指数”和相互作用自由能。还提出了新型的图，其中协同行为的类型比传统图中更明显。
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