Department of Biophysics, Basic Medical Sciences Block II, Panjab University, Chandigarh, India.
Centre for Nuclear Medicine (U.I.E.A.S.T), Panjab University, Chandigarh, India.
J Biochem Mol Toxicol. 2023 Nov;37(11):e23477. doi: 10.1002/jbt.23477. Epub 2023 Jul 21.
Malignant glioma is the deadliest form of brain cancer. Zingerone (ZO), a polyphenolic compound found in ginger, offers pharmacological properties that make it a promising agent for containing the growth of glioma cells. The present study was conducted to understand the efficacy of ZO in containing the growth of C6 glioma cells. The study also assessed the prophylactic role of ZO on rat brain glioma induced by C6 cell lines by addressing its antioxidative action on biochemical, behavioral, and histoarchitectural indices. For dose optimization, the animals were pretreated with different doses of ZO for a period of 2 weeks before the inoculation of glioma cells (1 × 10 /10 µL phosphate-buffered saline) in the caudate region of rat brain and the treatment with ZO continued for 4 more weeks post implantation. In vitro studies were done to assess the radical scavenging activity of ZO and also to determine its effects on viability of C6 glioma cells at different concentrations. Glioma-bearing rats showed significant alterations in memory; exploratory and muscular activities which were appreciably improved upon simultaneous supplementation of ZO administered at a dose of 50 mg/kg body weight and were also visible even at a higher dose. Glioma-bearing rats revealed a significant increase in reactive oxygen species, protein carbonyl contents, and lipid peroxidation, but showed a significant decrease in reduced glutathione and antioxidative enzymes in the brain tissue. Interestingly, all the biochemical indices and altered brain histoarchitecture displaying cellular atypia and hyperplasia showed appreciable improvement when supplemented with ZO at a dose of 50 mg/kg body weight.
恶性神经胶质瘤是最致命的脑癌形式。姜酮（ZO）是生姜中发现的一种多酚化合物，具有药理学特性，使其成为控制神经胶质瘤细胞生长的有前途的药物。本研究旨在了解 ZO 抑制 C6 神经胶质瘤细胞生长的功效。该研究还通过评估 ZO 在 C6 细胞系诱导的大鼠脑神经胶质瘤中的抗氧化作用，对其生化、行为和组织形态学指标进行了评估，研究了 ZO 的预防作用。为了进行剂量优化，在向大鼠脑尾状核接种神经胶质瘤细胞（1×10 个/10 μL 磷酸盐缓冲液）之前，用不同剂量的 ZO 预处理动物 2 周，并在植入后继续用 ZO 治疗 4 周。进行了体外研究，以评估 ZO 的自由基清除活性，并确定其在不同浓度下对 C6 神经胶质瘤细胞活力的影响。荷神经胶质瘤大鼠的记忆、探索和肌肉活动发生了显著变化，同时补充 50mg/kg 体重的 ZO 可显著改善这些变化，即使在较高剂量下也能观察到这些变化。荷神经胶质瘤大鼠的脑组织中活性氧、蛋白质羰基含量和脂质过氧化显著增加，但还原型谷胱甘肽和抗氧化酶显著减少。有趣的是，当用 50mg/kg 体重的 ZO 补充时，所有生化指标和改变的脑组织形态结构显示出细胞异型性和增生，都有明显改善。
