College of Biotechnology, Tianjin University of Science and Technology, Tianjin, P. R. China.
School of Life Sciences, Nanjing University, Nanjing, P. R. China.
J Food Sci. 2023 Sep;88(9):3649-3665. doi: 10.1111/1750-3841.16708. Epub 2023 Jul 21.
Plant constituents are of great interest in the food processing industry as potential natural preservative agents for controlling foodborne pathogens. In this study, the 95% EtOH/H O extract of Ginkgo biloba leaves was separated using polarity extraction solvents with petroleum ether (PE), ethyl acetate (EA), n-butanol (nB), and water (W) by the principle of similarity and compatibility. Through TLC and NMR analysis of these extracts, it can be concluded that the main component of PE extract were organic acids, for EA extract were flavonoids, for nB extract were phenylpropanoids, and water extract were oligosaccharides. Twelve monomer compounds were separated from the extracts to verify the composition of each extraction stage. Results of morphological and molecular identification revealed that Monilinia fructicola and Rhizopus stolonifer were the main fungi causing peach rot. After evaluating the antifungal activity and peach quality of the four extract/sodium alginate coatings, it was found that the n-butanol extract/sodium alginate coating containing phenylpropanoids had the lowest decay index and the best preservation effect, providing a sustainable alternative to reduce the harm to the environment of synthetic preservatives. PRACTICAL APPLICATION: The abuse of synthetic preservatives poses a threat to the ecological environment and physical health. Therefore, this study developed sodium alginate coating of Ginkgo biloba leaves extract containing phenylpropanoids, which has good effects on the preservation of peaches. The agent is a promising environmentally friendly alternative for synthetic preservatives.
植物成分在食品加工行业中备受关注,可作为控制食源性病原体的潜在天然防腐剂。在这项研究中,采用相似相溶原理,以石油醚(PE)、乙酸乙酯(EA)、正丁醇(nB)和水(W)作为极性萃取溶剂,对银杏叶 95%乙醇/水提取物进行分离。通过对这些提取物进行 TLC 和 NMR 分析,可以得出结论,PE 提取物的主要成分是有机酸,EA 提取物的主要成分是类黄酮,nB 提取物的主要成分是苯丙素,而水提取物的主要成分是低聚糖。从提取物中分离出 12 种单体化合物,以验证每个提取阶段的成分。形态学和分子鉴定结果表明,桃褐腐病菌和根霉是引起桃腐烂的主要真菌。评估了四种提取物/海藻酸钠涂层的抑菌活性和桃品质后发现,含有苯丙素的正丁醇提取物/海藻酸钠涂层的腐烂指数最低,具有最佳的保鲜效果,为减少合成防腐剂对环境的危害提供了一种可持续的替代方案。实际应用:合成防腐剂的滥用对生态环境和人体健康构成威胁。因此,本研究开发了含有苯丙素的银杏叶提取物海藻酸钠涂层,对桃的保鲜效果良好。该制剂是一种很有前途的、替代合成防腐剂的环保型替代品。