Department of Food Science and Technology, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China.
Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China.
Molecules. 2018 Dec 5;23(12):3214. doi: 10.3390/molecules23123214.
leaves have various health benefits due to the presence of bioactive compounds such as polyprenyl acetates, flavonoids, and terpene trilactones. However, there is little literature reported on the aromatic acids in leaves. In this work, five aromatic acids including shikimic acid (SA), 6-hydroxykynurenic acid (6-HKA), protocatechuic acid (PA), gallic acid (GAA), and -hydroxybenzoic acid (PHBA) were simultaneously extracted from leaves by employing the green deep eutectic solvents (DESs). A DES tailor-made from xylitol, glycolic acid and 1,5-pentanedioic acid at a molar ratio of 1:3:1 with 50% (/) water addition, named as NGG50, gave higher extraction yields for the five aromatic acids. Main factors affecting the extraction process were further optimized. The highest extraction yields of SA, GAA, 6-HKA, PA, and PHBA were 94.15 ± 0.96 mg/g, 332.69 ± 5.19 μg/g, 25.90 ± 0.61 μg/g, 429.89 ± 11.47 μg/g and 67.94 ± 0.37 μg/g, respectively. The NGG50-based extraction process developed here was a successful attempt of simultaneously extracting five aromatic acids from leaves for the first time, which could provide a new exploitation direction of leaves.
由于存在生物活性化合物，如聚异戊二烯乙酸酯、类黄酮和三萜内酯，叶子具有多种健康益处。然而，关于叶子中的芳香酸的文献报道很少。在这项工作中，通过使用绿色深共晶溶剂（DESs），从叶子中同时提取了五种芳香酸，包括莽草酸（SA）、6-羟基犬尿氨酸（6-HKA）、原儿茶酸（PA）、没食子酸（GAA）和-羟基苯甲酸（PHBA）。一种由木糖醇、乙醇酸和 1,5-戊二酸以摩尔比 1:3:1 制成的 DES，并添加了 50%（/）水，命名为 NGG50，对这五种芳香酸的提取率更高。进一步优化了影响提取过程的主要因素。SA、GAA、6-HKA、PA 和 PHBA 的最大提取率分别为 94.15±0.96mg/g、332.69±5.19μg/g、25.90±0.61μg/g、429.89±11.47μg/g 和 67.94±0.37μg/g。这里开发的基于 NGG50 的提取工艺首次成功地从叶子中同时提取了五种芳香酸，为叶子的开发提供了一个新的方向。
