National Key Laboratory of Materials for Integrated Circuits, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.
Anal Chem. 2023 Aug 1;95(30):11449-11455. doi: 10.1021/acs.analchem.3c01804. Epub 2023 Jul 21.
The blood-brain barrier (BBB) is essential for maintaining central nervous system (CNS) stability, and neuroinflammation may cause the dysfunction of the BBB. MicroRNA-146a (miR-146a) is closely associated with neuroinflammation, which showed significant upregulation in response to lipopolysaccharide (LPS) induction. Elucidating the relationship between LPS-induced miR-146a expression and the BBB could decipher the mechanism of many neurological diseases. Here, we constructed an in vitro microfluidic human-BBB (μF-hBBB) chip consisting of human umbilical vein vascular endothelial cells (HUVECs) and human astrocyte (HAs) cells. A tetrahedral DNA framework (TDF-3MB) nanoprobe was used to label miR-146a in HUVECs on μF-hBBB chips before and after LPS induction, and the study revealed a significant increase in miR-146a expression after LPS induction. We believe that such a μF-hBBB chip is a promising in vitro platform for further use in understanding CNS diseases.
血脑屏障（BBB）对于维持中枢神经系统（CNS）的稳定性至关重要，而神经炎症可能导致 BBB 功能障碍。微小 RNA-146a（miR-146a）与神经炎症密切相关，其表达在脂多糖（LPS）诱导下显著上调。阐明 LPS 诱导的 miR-146a 表达与 BBB 之间的关系可以揭示许多神经疾病的发病机制。在这里，我们构建了一种由人脐静脉血管内皮细胞（HUVECs）和人星形胶质细胞（HAs）组成的体外人 BBB（μF-hBBB）芯片。使用四面体 DNA 框架（TDF-3MB）纳米探针在 LPS 诱导前后标记 μF-hBBB 芯片上的 HUVECs 中的 miR-146a，研究表明 LPS 诱导后 miR-146a 的表达显著增加。我们相信，这种 μF-hBBB 芯片是一种很有前途的体外平台，可进一步用于了解 CNS 疾病。
