Masuda K, Kawata T
Microbiol Immunol. 1986;30(5):401-11. doi: 10.1111/j.1348-0421.1986.tb02966.x.
An outer sheath was isolated from Treponema phagedenis biotype Reiter by our previously developed method (Masuda, K., and Kawata, T. 1982. J. Bacteriol. 150: 1405-1413). The isolated outer sheath was observed as a triple-layered, closed vesicle carrying a polygonal array by electron microscopy. The outer sheath was mainly composed of protein (41.0%), phospholipid (38.7%), and carbohydrate (11.0%). Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the isolated outer sheath in the presence of 2-mercaptoethanol (EtSH) gave one major protein band with an apparent molecular weight of about 69,000 and several minor protein bands. On the other hand, in the absence of EtSH, the major protein band disappeared but two new protein bands at positions of molecular weights of about 65,000 and 72,000 appeared. The SDS-PAGE profiles of the minor protein bands did not change with or without EtSH. Sodium deoxycholate (DOC)-solubilized materials from the isolated outer sheath were reassembled into thin membranous sheets carrying a roughly polygonal array upon removal of DOC by dialysis against Tris-HCl buffer in the absence of Mg2+.
通过我们之前开发的方法（增田，K.，和川田，T. 1982.《细菌学杂志》150: 1405 - 1413），从蚀疮艾肯菌生物型赖特氏菌中分离出了外鞘。通过电子显微镜观察，分离出的外鞘呈现为带有多边形阵列的三层封闭囊泡。外鞘主要由蛋白质（41.0%）、磷脂（38.7%）和碳水化合物（11.0%）组成。在2 - 巯基乙醇（EtSH）存在的情况下，对分离出的外鞘进行十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE），得到一条表观分子量约为69,000的主要蛋白带和几条次要蛋白带。另一方面，在没有EtSH的情况下，主要蛋白带消失，但出现了两条分子量约为65,000和72,000的新蛋白带。次要蛋白带的SDS - PAGE图谱在有或没有EtSH的情况下都没有变化。从分离出的外鞘中用脱氧胆酸钠（DOC）溶解的物质，在不存在Mg2 + 的情况下，通过用Tris - HCl缓冲液透析去除DOC后，重新组装成带有大致多边形阵列的薄膜片。
