State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, and Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China; College of Food Science and Technology, Guangdong Ocean University, Yangjiang Campus, Yangjiang, 529500, China.
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, and Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China; Department of Food and Environmental Engineering, Yangjiang Polytechnic, Yangjiang, 529500, China.
Theriogenology. 2023 Oct 15;210:62-67. doi: 10.1016/j.theriogenology.2023.07.016. Epub 2023 Jul 16.
The cryopreservation process is associated with the generation of excessive reactive oxygen species, which causes a series of cellular damage to spermatozoa. The objective of the current study was to investigate the effect of different concentrations of cysteine on post-thaw sperm quality of brown-marbled grouper sperm. Semen samples were frozen with cysteine supplemented at 0.5, 1, 2, 5, 10 mM and the control group (no additive). After thawing, sperm quality parameters were analyzed. In comparison to the control, cysteine treatment groups yielded relatively higher sperm total motility, progressive motility, and curvilinear velocity. Different concentrations of cysteine had no effect on average path velocity, straight linear velocity and viability (P > 0.05), while an increase in the concentration of cysteine resulted in a significant improvement in the mitochondrial membrane potential, SOD activity, and ATP content (P < 0.05). As for lipid peroxidation, the extent of which in cysteine treated spermatozoa was less than the control, although the differences were not statistically significant (P > 0.05). In terms of fertilizing capacity, a greater hatching rate (91.7 ± 1.2%) was obtained in thawed sperm treated with 2 mM cysteine, compared to the control (84.3 ± 4.2%; P < 0.05). Overall, it is concluded that the addition of cysteine is helpful in maintaining the function of frozen-thawed brown-marbled grouper sperm, which can be recommended as an effective antioxidant to improve the semen cryopreservation efficiency.
冷冻保存过程会产生大量的活性氧，导致精子发生一系列细胞损伤。本研究旨在探讨不同浓度半胱氨酸对褐点石斑鱼精子解冻后精子质量的影响。用添加 0.5、1、2、5、10 mM 半胱氨酸和对照组（无添加剂）的方法对精液样本进行冷冻，然后解冻分析精子质量参数。与对照组相比，半胱氨酸处理组的精子总活力、前向运动活力和曲线速度较高。不同浓度的半胱氨酸对平均路径速度、直线速度和活力没有影响（P>0.05），但随着半胱氨酸浓度的增加，线粒体膜电位、SOD 活性和 ATP 含量显著提高（P<0.05）。对于脂质过氧化，虽然半胱氨酸处理的精子中的脂质过氧化程度低于对照组，但差异无统计学意义（P>0.05）。在受精能力方面，用 2 mM 半胱氨酸处理的解冻精子的孵化率（91.7±1.2%）显著高于对照组（84.3±4.2%；P<0.05）。总之，添加半胱氨酸有助于维持冷冻-解冻褐点石斑鱼精子的功能，可作为一种有效的抗氧化剂来提高精液冷冻保存效率。
