College of Food Science and Technology, Guangdong Ocean University (Yangjiang Campus), Yangjiang, 529500, China.
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, and Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China; Department of Food and Environmental Engineering, Yangjiang Polytechnic, Yangjiang, 529500, China.
Theriogenology. 2021 Nov;175:77-82. doi: 10.1016/j.theriogenology.2021.08.029. Epub 2021 Aug 26.
Incorporation of Coenzyme Q10 (CoQ10) to the freezing medium provides advantageous effect for sperm cryopreservation in a variety of animal species, yet which has not been tested in giant grouper (Epinephelus lanceolatus). This research was designed to elucidate if CoQ10 could be used as a potential additive to improve giant grouper sperm quality after cryopreservation. After the process of freezing and thawing, various sperm quality parameters including motility, viability, apoptosis, mitochondrial membrane potential (MMP), intracellular reactive oxygen species (ROS) generation, DNA fragmentation as well as fertilization rate were evaluated with CoQ10 added at concentrations of 0, 25, 50 and 100 μM. Compared to the control group (0 μm), addition of CoQ10 in the medium yielded significantly higher total motility and curvilinear velocity, whereas the progressive motility, straight-line velocity and average path velocity were not differ from each other. An obvious improvement in viability was observed in spermatozoa cryopreserved with 25 and 50 μM CoQ10, while the apoptosis rate in CoQ10 treated groups (25, 50 and 100 μM) exhibited significantly lower values than that of the control. Besides, the production of ROS was significantly decreased with CoQ10 addition groups when compared with the control. In consistent with the improvement in antioxidant defense, CoQ10 supplementation in the medium also enhanced mitochondrial activity and reduced DNA fragmentation. In addition, freezing medium supplemented with CoQ10 also improved the fertilization success, a significantly higher fertilization rate was recorded at the concentration of 50 μM, but this value was not differ from that of 25 μM. Overall, the antioxidant CoQ10 provided an obvious beneficial effect on post-thaw quality of giant grouper spermatozoa. It was concluded that the optimal concentration of CoQ10 is 50 μM in the freezing medium.
辅酶 Q10（CoQ10）在冷冻液中的添加对多种动物物种的精子冷冻保存具有有利影响，但尚未在巨石斑鱼（Epinephelus lanceolatus）中进行测试。本研究旨在阐明 CoQ10 是否可用作潜在添加剂来改善冷冻保存后的巨石斑鱼精子质量。在冷冻和解冻过程后，通过添加浓度为 0、25、50 和 100 μM 的 CoQ10 来评估各种精子质量参数，包括活力、活力、凋亡、线粒体膜电位（MMP）、细胞内活性氧（ROS）生成、DNA 片段化和受精率。与对照组（0 μm）相比，在培养基中添加 CoQ10 可显著提高总活力和曲线速度，而直线速度和平均路径速度则没有差异。在添加 25 和 50 μM CoQ10 的冷冻精子中观察到活力明显提高，而 CoQ10 处理组（25、50 和 100 μM）的凋亡率显著低于对照组。此外，与对照组相比，添加 CoQ10 可显著降低 ROS 的产生。与抗氧化防御的改善一致，在培养基中添加 CoQ10 还增强了线粒体活性并减少了 DNA 片段化。此外，添加 CoQ10 的冷冻液还提高了受精成功率，在 50 μM 时记录到明显更高的受精率，但与 25 μM 时的受精率没有差异。总体而言，抗氧化剂 CoQ10 对巨石斑鱼精子解冻后的质量有明显的有益作用。结论是，冷冻液中 CoQ10 的最佳浓度为 50 μM。
