Hebei Key Laboratory of Medical-Industrial Integration Precision Medicine, College of Chemical Engineering, North China University of Science and Technology, Tangshan 063210, China.
National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
J Phys Chem B. 2024 Apr 25;128(16):3910-3918. doi: 10.1021/acs.jpcb.4c00161. Epub 2024 Apr 12.
Viscosity, at the subcellular level, plays a crucial role as a physicochemical factor affecting microenvironment homeostasis. Abnormal changes in mitochondrial viscosity often lead to various diseases in the organism. Based on the twisted intramolecular charge transfer mechanism, four hemicyanine dye fluorescent probes (, , , and ) were designed and synthesized for viscosity response. The single bond between the nitrogen-containing heterocycle and the carbon-carbon double in the structure of the probe bond served as the viscosity response site. Finally, the probe was screened as the optimal mitochondrial viscosity probe according to its responsiveness, targeting, and interference resistance. The fluorescence intensity of the probe HT-Bzh increased 22-fold when the viscosity was increased from 13.75 to 811.2 cP. In summary, all four viscosity probes we have developed can be used in different applications depending on the external environment, providing a valuable reference for the design of potential tools to address viscosity monitoring in biological systems.
黏度在亚细胞水平上作为影响微环境稳态的物理化学因素起着至关重要的作用。线粒体黏度的异常变化往往会导致生物体的各种疾病。基于扭曲的分子内电荷转移机制，设计并合成了四个半花菁染料荧光探针（、、、和）用于响应黏度变化。探针结构中氮杂环和碳-碳双键之间的单键作为黏度响应位点。最后，根据其响应性、靶向性和抗干扰性，筛选出探针 HT-Bzh 作为最佳线粒体黏度探针。当黏度从 13.75 增加到 811.2 cP 时，探针 HT-Bzh 的荧光强度增加了 22 倍。总之，我们开发的所有四个黏度探针都可以根据外部环境在不同的应用中使用，为设计用于监测生物系统黏度的潜在工具提供了有价值的参考。
