Liu Feng-Ting, Wang Shuo, Wang Yan-Pu, Jiang Peng-Fei, Miao Jun-Ying, Zhao Bao-Xiang, Lin Zhao-Min
Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
Institute of Developmental Biology, School of Life Science, Shandong University, Qingdao 266237, PR China.
Talanta. 2024 Aug 1;275:126135. doi: 10.1016/j.talanta.2024.126135. Epub 2024 Apr 24.
Hydrogen peroxide (HO) and viscosity play vital roles in the cellular environment as signaling molecule and microenvironment parameter, respectively, and are associated with many physiological and pathological processes in biological systems. We developed a near-infrared fluorescent probe, CQ, which performed colorimetric and ratiometric detection of HO and viscosity based on the FRET mechanism, and was capable of monitoring changes in viscosity and HO levels simultaneously through two different channels. Based on the specific reaction of HO with borate ester, CQ exhibited a significant ratiometric response to HO with a large Stokes shift of 221 nm, a detection limit of 0.87 μM, a near-infrared emission wavelength of 671 nm, a response time of 1 h, a wide detection ranges of 0.87-800 μM and a high energy transfer efficiency of 99.9 %. CQ could also recognize viscosity by the TICT mechanism, and efficiently detect viscosity changes caused by food thickeners. More importantly, CQ could successfully detect endogenous/exogenous HO and viscosity in live HeLa cells, which was expected to be a practical tool for detecting HO and viscosity in live cells.
过氧化氢（HO）和黏度在细胞环境中分别作为信号分子和微环境参数发挥着至关重要的作用，并且与生物系统中的许多生理和病理过程相关。我们开发了一种近红外荧光探针CQ，它基于荧光共振能量转移（FRET）机制对HO和黏度进行比色和比率检测，并且能够通过两个不同通道同时监测黏度和HO水平的变化。基于HO与硼酸酯的特异性反应，CQ对HO表现出显著的比率响应，斯托克斯位移为221 nm，检测限为0.87 μM，近红外发射波长为671 nm，响应时间为1小时，检测范围宽达0.87 - 800 μM，能量转移效率高达99.9%。CQ还可通过扭曲分子内电荷转移（TICT）机制识别黏度，并能有效检测由食品增稠剂引起的黏度变化。更重要的是，CQ能够成功检测活HeLa细胞内的内源性/外源性HO和黏度，有望成为一种检测活细胞中HO和黏度的实用工具。
