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抗瓜氨酸化蛋白抗体在系统性硬化症中的作用。

Anti-carbamylated protein antibodies in systemic sclerosis.

机构信息

Department of Rheumatology, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The Netherlands.

出版信息

Arthritis Res Ther. 2023 Jul 21;25(1):123. doi: 10.1186/s13075-023-03099-3.

DOI:10.1186/s13075-023-03099-3

PMID:37480150

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10360323/

Abstract

BACKGROUND

To investigate the presence of different isotypes of anti-carbamylated protein (CarP) antibodies in systemic sclerosis (SSc) patients and its association with skin involvement.

METHODS

Sera of 194 SSc patients from the Leiden CCISS cohort, fulfilling ACR/EULAR 2013 criteria and a clinical diagnosis of SSc, 83 patients with other connective tissue diseases/Raynaud's Phenomenon, 24 rheumatoid arthritis patients and 98 age and sex-matched healthy controls were tested for the presence of anti-CarP IgG, IgA and IgM, determined by ELISA. Clinical characteristics, that were evaluated in SSc patients, included age, anti-topoisomerase antibodies (ATA), anti-centromere antibodies (ACA) and modified Rodnan Skin Score (mRSS).

RESULTS

The SSc patients were 55 (SD:13) years and 155 (80%) were female. Forty-four (23%) patients tested positive for ATA, and 80 (42%) ACA. The median mRSS was 2 (range: 0; 47). Prevalence of anti-CarP IgG was higher in SSc patients than in healthy controls (8% vs 3%, p = 0.007. Prevalence of anti-CarP IgA and IgM and levels of anti-CarP isotypes were comparable between SSc patients and healthy controls. Fifteen (8%) SSc patients tested positive for anti-CarP IgG, 16 (8%) for anti-CarP IgA, and 36 (19%) for anti-CarP IgM. There were no significant correlations between age and levels of anti-CarP isotypes. No correlation between anti-CarP IgG levels and mRSS was found (r = 0.141, p = 0.049), nor for anti-CarP IgM and IgA levels. Anti-CarP IgA levels were higher in ATA compared to ACA positive SSc patients (ATA: 616 aU/ml [359; 1103]; ACA: 424 aU/ml [300; 673], p = 0.015).

CONCLUSION

SSc patients can test positive for Anti-CarP IgG, IgA and IgM. We do not observe a relevant clinical association between anti-CarP antibody response and skin involvement in SSc.

摘要

背景

为了研究系统性硬化症（SSc）患者中不同类型的抗氨甲酰化蛋白（CarP）抗体的存在情况及其与皮肤受累的关系。

方法

194 名来自莱顿 CCISS 队列的 SSc 患者血清，符合 ACR/EULAR 2013 标准和 SSc 的临床诊断，83 名其他结缔组织疾病/雷诺现象患者，24 名类风湿关节炎患者和 98 名年龄和性别匹配的健康对照者接受抗 CarP IgG、IgA 和 IgM 的检测，采用 ELISA 法。在 SSc 患者中评估的临床特征包括年龄、抗拓扑异构酶抗体（ATA）、抗着丝粒抗体（ACA）和改良 Rodnan 皮肤评分（mRSS）。

结果

SSc 患者的平均年龄为 55（标准差：13）岁，155 名（80%）为女性。44 名（23%）患者检测到 ATA 阳性，80 名（42%）患者 ACA 阳性。mRSS 的中位数为 2（范围：0；47）。SSc 患者的抗 CarP IgG 阳性率高于健康对照组（8% vs 3%，p=0.007）。SSc 患者与健康对照组之间抗 CarP IgA 和 IgM 的阳性率和抗 CarP 同种型水平相当。15 名（8%）SSc 患者抗 CarP IgG 阳性，16 名（8%）抗 CarP IgA 阳性，36 名（19%）抗 CarP IgM 阳性。年龄与抗 CarP 同种型水平之间无显著相关性。未发现抗 CarP IgG 水平与 mRSS 之间存在相关性（r=0.141，p=0.049），也未发现抗 CarP IgM 和 IgA 水平与 mRSS 之间存在相关性。与 ACA 阳性的 SSc 患者相比，ATA 阳性的 SSc 患者抗 CarP IgA 水平更高（ATA：616 aU/ml [359；1103]；ACA：424 aU/ml [300；673]，p=0.015）。