聚（dI）·聚（dC）和聚（rI）·聚（dC）的免疫原性——单克隆抗体分析

The immunogenic properties of poly(dI).poly(dC) and poly(rI). poly(dC)--analysis by monoclonal antibodies.

作者信息

Braun R P, Woodsworth M L, Lee J S

出版信息

Mol Immunol. 1986 Jun;23(6):685-91. doi: 10.1016/0161-5890(86)90107-0.

Abstract

Eight monoclonal antibodies were prepared from a C57/black mouse which had been immunized with poly(rI).poly(dC). Two of the antibodies were specific for the RNA-DNA duplex but bound about 100-fold better to poly(rI).poly(dC) than to poly(rA).poly(dT). The other six antibodies were single-strand specific and bound to poly(rI) and in most cases to poly(dI) as well, but not to other single-stranded nucleic acids. Similarly, 10 monoclonal antibodies were produced from mice immunized with poly(dI).poly(dC). Nine of these were specific for poly(dI) while the other had a strong preference for poly(dC). Thus, in contrast to poly(rI).poly(dC), the all DNA duplex poly(dI). poly(dC) only elicits duplex specific antibodies at very low frequency, if at all.

摘要

用聚（rI）·聚（dC）免疫C57/黑小鼠，制备了8种单克隆抗体。其中两种抗体对RNA-DNA双链体具有特异性，但与聚（rI）·聚（dC）的结合能力比对聚（rA）·聚（dT）的结合能力强约100倍。另外六种抗体是单链特异性的，与聚（rI）结合，大多数情况下也与聚（dI）结合，但不与其他单链核酸结合。同样，用聚（dI）·聚（dC）免疫小鼠产生了10种单克隆抗体。其中9种对聚（dI）具有特异性，另一种则强烈偏好聚（dC）。因此，与聚（rI）·聚（dC）不同，全DNA双链体聚（dI）·聚（dC）即使能诱导产生双链特异性抗体，频率也非常低。