Guangdong Technology Research Center for Traditional Chinese Veterinary Medicine and Natural Medicine, South China Agricultural University, Guangzhou, 510642, China.
Qilu Animal Health Products Company, Jinan, 250033, China.
Curr Med Sci. 2023 Aug;43(4):831-837. doi: 10.1007/s11596-022-2647-8. Epub 2023 Jul 22.
Polyphenols are complex compounds containing multiple phenolic hydroxyl groups. They are widely distributed in plants and have antioxidant activities. Whether the antioxidant activities of the cultivated varieties of Echinacea are similar to or better than those of the wild ones and the relationship between the accumulation of polyphenols and their antioxidant activities are still not clear.
Folin-Ciocalteu method, high performance liquid chromatography (HPLC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, ferric ion reducing antioxidant power (FRAP) assay, 2,2'-azino-bis(3-ethylbenzothiazoline-6)-sulfonic acid (ABTS) radical scavenging assay, and Fe chelating ability assay were used, respectively, to detect the total polyphenols and 5 kinds of caffeic acid derivatives (chicoric acid, caffeic acid, caftaric acid, chlorogenic acid, and 1,5-dicaffeoylquinic acid) in the roots, stems, leaves, and flowers, and the antioxidant activities of 3 varieties of Echinacea: E. purpurea L., cultivar E. purpurea 'Aloha', and E. purpurea 'White Swan'.
E. purpurea L. had the highest contents of total polyphenols, 5 caffeic acid derivatives and antioxidant activities, followed by E. purpurea 'White Swan' and E. purpurea 'Aloha', respectively. E. purpurea 'White Swan' had the strongest ability to remove the DPPH, ABTS• and free radicals, and to chelate Fe; E. purpurea L. had the strongest ability to reduce FRAP. The correlation analyses revealed that the contents of total polyphenols and caffeic acid derivatives of E. purpurea L. and E. purpurea 'White Swan' were correlated with their antioxidant activities.
E. purpurea L. was the most appropriate material for the development of medicinal plants. E. purpurea 'White Swan' could be used as a substitute for E. purpurea L. in terms of its antioxidant activity.
多酚是含有多个酚羟基的复杂化合物。它们广泛存在于植物中，具有抗氧化活性。栽培品种的紫锥菊的抗氧化活性是否与野生品种相似或更好，以及多酚的积累与其抗氧化活性之间的关系尚不清楚。
采用福林-考卡尔特乌（Folin-Ciocalteu）法、高效液相色谱法（HPLC）、2,2-二苯基-1-苦基肼（DPPH）自由基清除法、铁还原抗氧化能力（FRAP）法、2,2'-联氮-双（3-乙基苯并噻唑啉-6）-磺酸（ABTS）自由基清除法和Fe 螯合能力测定法，分别检测根、茎、叶、花中总多酚和 5 种咖啡酸衍生物（菊苣酸、咖啡酸、卡塔酸、绿原酸和 1,5-二咖啡酰奎宁酸），以及 3 种紫锥菊品种：E. purpurea L.、栽培品种 E. purpurea 'Aloha'和 E. purpurea 'White Swan'的抗氧化活性。
E. purpurea L.的总多酚、5 种咖啡酸衍生物和抗氧化活性含量最高，其次是 E. purpurea 'White Swan'和 E. purpurea 'Aloha'。E. purpurea 'White Swan'清除 DPPH、ABTS•和自由基以及螯合 Fe 的能力最强；E. purpurea L.还原 FRAP 的能力最强。相关性分析表明，E. purpurea L.和 E. purpurea 'White Swan'总多酚和咖啡酸衍生物的含量与其抗氧化活性相关。
E. purpurea L.是开发药用植物最适宜的材料。E. purpurea 'White Swan'在抗氧化活性方面可以替代 E. purpurea L.。
