Programa de Pós-Graduação em Biologia Celular e Molecular, Escola de Ciências da Saúde e da Vida, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Laboratório de Farmacologia Aplicada, Escola de Ciências da Saúde e da Vida, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Naunyn Schmiedebergs Arch Pharmacol. 2024 Jan;397(1):521-534. doi: 10.1007/s00210-023-02617-z. Epub 2023 Jul 22.
The danger of ionizing radiation exposure to human health is a concern. Since its wide use in medicine and industry, the development of radioprotectors has been very significant. Adenosine exerts anti-inflammatory actions and promotes tissue protection and repair, by activating the P1 receptors (A, A, A, and A). Zebrafish (Danio rerio) is an appropriate tool in the fields of toxicology and pharmacology, including the evaluation of radiobiological outcomes and in the search for radioprotector agents. This study aims to evaluate the effect of adenosine in the toxicity induced by radiation in zebrafish. Embryos were treated with 1, 10, or 100 µM adenosine, 30 min before the exposure to 15 Gy of gamma radiation. Adenosine potentiated the effects of radiation in heart rate, body length, and pericardial edema. We evaluated oxidative stress, tissue remodeling and inflammatory. It was seen that 100 µM adenosine reversed the inflammation induced by radiation, and that A and A receptors are involved in these anti-inflammatory effects. Our results indicate that P1R activation could be a promising pharmacological strategy for radioprotection.
电离辐射对人类健康的危害令人担忧。自其在医学和工业中的广泛应用以来，放射防护剂的发展非常重要。腺苷通过激活 P1 受体（A、A、A 和 A）发挥抗炎作用，并促进组织保护和修复。斑马鱼（Danio rerio）是毒理学和药理学领域的一种合适工具，包括评估放射生物学结果和寻找放射防护剂。本研究旨在评估腺苷对斑马鱼辐射诱导毒性的影响。胚胎在暴露于 15Gyγ射线前 30 分钟用 1、10 或 100µM 腺苷处理。腺苷增强了心率、体长和心包水肿的辐射效应。我们评估了氧化应激、组织重塑和炎症。结果表明，100µM 腺苷逆转了辐射引起的炎症，A 和 A 受体参与了这些抗炎作用。我们的结果表明，P1R 激活可能是一种有前途的放射防护药理学策略。
