Engineering and Technology Research Center for Waterfowl Resources Development and Utilization and Epidemic Disease Prevention and Control of Henan Province, Xinyang Agriculture and Forestry University, Xinyang, Henan Province, 464000, PR China.
Engineering and Technology Research Center for Waterfowl Resources Development and Utilization and Epidemic Disease Prevention and Control of Henan Province, Xinyang Agriculture and Forestry University, Xinyang, Henan Province, 464000, PR China.
Theriogenology. 2023 Oct 15;210:84-93. doi: 10.1016/j.theriogenology.2023.06.037. Epub 2023 Jul 7.
Preservation of ovarian tissues is an effective way to ensure genetic diversity of susceptible natural bird populations that are in danger of extinction. We examined whether the addition of the plant phenol resveratrol to vitrification solutions ameliorates the damaging effects of tissue hypoxia and reperfusion injury when the tissues are transplanted. Duck ovary tissues were frozen in the presence of varying concentrations of resveratrol in cryopreservation solutions and then transplanted under the renal capsules of 2-day-old Shelducks. Samples of the transplanted tissues were examined on days 3- and 9- post transplantation for activation of hypoxia-, antioxidant- and apoptosis-related gene expression and apoptosis. Resveratrol significantly increased expression of VEGF, HIF-1α, Nrf2, CAT and Bcl-2 mRNA and decreased BAX and Caspase-3 mRNA and reduced numbers of TUNEL-positive cells after vitrification and heterotopic ovarian transplantation. Resveratrol improved the antioxidant capacity, reduced apoptosis and activated the HIF-1α/VEGF pathway to promote angiogenesis 3- and 9-days following transplantation. These results indicated that the addition of resveratrol to vitrification solutions intended for long-term cryopreservation of ovary tissues improves survival in storage and the grafts following transplantation. This study provides a theoretical basis for the successful transplantation of avian ovarian tissue after vitrification.
卵巢组织的保存是确保处于灭绝危险的易感性自然鸟类种群遗传多样性的有效方法。我们研究了当组织移植时，向玻璃化溶液中添加植物酚白藜芦醇是否可以改善组织缺氧和再灌注损伤的破坏性影响。鸭卵巢组织在含有不同浓度白藜芦醇的冷冻保护溶液中冷冻，然后移植到 2 日龄 Shelducks 的肾包膜下。在移植后第 3 天和第 9 天，对移植组织的样本进行检查，以检测缺氧、抗氧化和凋亡相关基因表达和凋亡的激活情况。白藜芦醇显著增加了 VEGF、HIF-1α、Nrf2、CAT 和 Bcl-2mRNA 的表达，降低了 BAX 和 Caspase-3mRNA 的表达，并减少了 TUNEL 阳性细胞的数量，在玻璃化和异位卵巢移植后。白藜芦醇提高了抗氧化能力，减少了凋亡，并激活了 HIF-1α/VEGF 通路，促进了移植后 3 天和 9 天的血管生成。这些结果表明，向旨在长期冷冻保存卵巢组织的玻璃化溶液中添加白藜芦醇可以提高储存和移植后移植物的存活率。这项研究为禽类卵巢组织玻璃化后成功移植提供了理论依据。
