Cellulose and Paper Department, National Research Centre, Cairo, P.O. 12622, Egypt.
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt.
Int J Biol Macromol. 2023 Sep 1;248:125872. doi: 10.1016/j.ijbiomac.2023.125872. Epub 2023 Jul 22.
Nowadays, the combined knowledge and experience in biomedical research and material sciences results in the innovation of smart materials that could efficiently overcome the problems of microbial contaminations. Herein, a new drug delivery platform prepared by grafting sodium alginate with β-carboxyethyl acrylate and acrylamide was described and characterized. 9-Aminoacridine (9-AA), and kanamycin sulfate (KS) were separately loaded into the hydrogel in situ during graft polymerization. The grafting efficiency for the resulting hydrogels was 70.01-78.08 %. The chemical structure of the hydrogels, thermogravimetric analysis, and morphological features were investigated. The swelling study revealed that the hydrogel without drugs achieved a superior swelling rate compared to drug-loaded hydrogels. The hydrogel tuned the drug-release rate in a pH-dependent manner. Furthermore, the antibacterial study suggested that the hydrogels encapsulating 9-AA (88.6 %) or KS (89.3 %) exhibited comparable antibacterial activity against Gram-positive and Gram-negative bacterial strains. Finally, the cytocompatibility study conducted on normal lung cell line (Vero cells) demonstrated neglectable to tolerable toxicity for the drug-loaded hydrogel. More interestingly, the cell viability for the blank hydrogel was 92.5 %, implying its suitability for biomedical applications.
如今，生物医学研究和材料科学方面的综合知识和经验催生了智能材料的创新，这些材料可以有效地克服微生物污染问题。在此，描述并表征了通过将海藻酸钠与β-羧乙基丙烯酰胺和丙烯酰胺接枝制备的新型药物输送平台。9-氨基吖啶（9-AA）和硫酸卡那霉素（KS）分别在接枝聚合过程中原位载入水凝胶中。所得水凝胶的接枝效率为 70.01-78.08%。对水凝胶的化学结构、热重分析和形态特征进行了研究。溶胀研究表明，无药物的水凝胶的溶胀率优于载药水凝胶。水凝胶以 pH 值依赖的方式调节药物释放速率。此外，抗菌研究表明，包封 9-AA（88.6%）或 KS（89.3%）的水凝胶对革兰氏阳性和革兰氏阴性细菌菌株表现出相当的抗菌活性。最后，在正常肺细胞系（Vero 细胞）上进行的细胞相容性研究表明，载药水凝胶的毒性可忽略不计或可耐受。更有趣的是，空白水凝胶的细胞活力为 92.5%，这意味着其适用于生物医学应用。
