Center for Natural and Human Sciences (CCNH), Universidade Federal do ABC (UFABC), CEP 09210-580, Santo André, São Paulo, Brazil.
Department of Microbiology, Universidade Estadual de Londrina (UEL), Campus Universitário, CEP 86055-990, Londrina, Paraná, Brazil.
ACS Biomater Sci Eng. 2020 Apr 13;6(4):2117-2134. doi: 10.1021/acsbiomaterials.9b01685. Epub 2020 Mar 5.
Nitric oxide (NO) and silver nanoparticles (AgNPs) are well-known for their antibacterial activity. In this work, S-nitroso-mercaptosuccinic acid (S-nitroso-MSA), a NO donor, and green tea synthesized AgNPs were individually or simultaneously incorporated into alginate hydrogel for topical antibacterial applications. The obtained hydrogels were characterized and the NO release and diffusion of AgNPs and S-nitroso-MSA from alginate hydrogels were investigated. The hydrogels showed a concentration dependent toxicity toward Vero cells. The potent antibacterial effect of the hydrogels was demonstrated toward ATCC 25922, ATCC 25923, and UA159. Interestingly, the combination of S-nitroso-MSA and AgNPs into alginate hydrogels had a superior antibacterial effect, compared with hydrogels containing S-nitroso-MSA or AgNPs individually. This is the first report to describe the synthesis, cytotoxicity, and antibacterial effects of alginate hydrogel containing NO donor and AgNPs. These hydrogels might find important local applications in the combat of bacterial infections.
一氧化氮（NO）和银纳米粒子（AgNPs）以其抗菌活性而闻名。在这项工作中，一氧化氮供体 S-亚硝基巯基琥珀酸（S-nitroso-MSA）和绿茶合成的 AgNPs 分别或同时被掺入藻酸盐水凝胶中，用于局部抗菌应用。所得水凝胶进行了表征，并研究了 NO 从藻酸盐水凝胶中的释放和 AgNPs 和 S-nitroso-MSA 的扩散。水凝胶对 Vero 细胞表现出浓度依赖性毒性。水凝胶对 ATCC 25922、ATCC 25923 和 UA159 表现出强大的抗菌作用。有趣的是，与单独含有 S-nitroso-MSA 或 AgNPs 的水凝胶相比，将 S-nitroso-MSA 和 AgNPs 组合到藻酸盐水凝胶中具有更好的抗菌效果。这是首次描述含有 NO 供体和 AgNPs 的藻酸盐水凝胶的合成、细胞毒性和抗菌作用的报告。这些水凝胶可能在对抗细菌感染方面具有重要的局部应用价值。
