Department of Microbiology and Immunology, Helwan University, Cairo,Egypt.
Department of Microbiology and Immunology, Helwan University, Cairo,EgyptSTIKes Kepanjen, Malang, Indonesia.
J Pak Med Assoc. 2023 Apr;73(Suppl 4)(4):S167-S173. doi: 10.47391/JPMA.EGY-S4-34.
To characterise the biofilm matrix composition of a newly described Staphylococcus aureus biofilm phenotype.
This experimental study was conducted at the Faculty of Pharmacy, Helwan University, Cairo, Egypt, from January 2021 to March 2022, and comprised methicillin-resistant Staphylococcus aureus and methicillin-susceptible Staphylococcus aureus biofilm-forming clinical isolates which were allowed to construct biofilms under two distinct culture conditions; one a commonly used condition, and the other one a novel, more biologically-relevant condition. The formed biofilms were analysed for matrix composition through treatment with proteinase,sodium meta-periodate, and streptokinase. The efficacy of Cis-2-Decenoic acid and hamamelitannin on the biologically-relevant biofilms was evaluated using biofilm viability assay based on a colorimetric assay for measuring cell metabolic activity and scanning electron microscope imaging. Data was analysed using GraphPad Prism 5.01.
Of the 58 isolates, 45(77.6%) were methicillin-resistant Staphylococcus aureus and 13(22.4%) were methicillin susceptible Staphylococcus aureus. There was significant difference in responses to streptokinase, proteinase and sodium meta-periodate (p<0.05) among the differentially-developed biofilms in methicillin-resistant Staphylococcus aureus isolates. Regarding the methicillin-susceptible Staphylococcus aureus isolates, the differentially-developed biofilms showed significantly different liabilities to streptokinase only (p<0.05). Mean biofilm inhibition for Cis-2- Decenoic acid was 54.27±27.93% and mean biofilm dispersion was 71.92±11.59% while the corresponding valuesfor hamamelitannin were 83.03±13.95% and 70.48±7.116% against the newly described methicillin-resistant Staphylococcus aureus biofilm phenotype.
Applying biologically-relevant culture conditions on staphylococci biofilms and antibiofilm drugs is recommended.
描述一种新描述的金黄色葡萄球菌生物膜表型的生物膜基质组成。
本实验研究于 2021 年 1 月至 2022 年 3 月在埃及开罗 Helwan 大学药学院进行，包括耐甲氧西林金黄色葡萄球菌和甲氧西林敏感金黄色葡萄球菌生物膜形成的临床分离株，这些分离株在两种不同的培养条件下形成生物膜；一种是常用条件，另一种是更具生物学相关性的新型条件。通过用蛋白酶、过碘酸钠和链激酶处理来分析形成的生物膜的基质组成。使用基于比色法测量细胞代谢活性的生物膜活力测定法和扫描电子显微镜成像来评估 Cis-2-癸烯酸和金缕梅单宁对新型相关生物膜的效果。使用 GraphPad Prism 5.01 分析数据。
在 58 株分离株中，45 株（77.6%）为耐甲氧西林金黄色葡萄球菌，13 株（22.4%）为甲氧西林敏感金黄色葡萄球菌。耐甲氧西林金黄色葡萄球菌分离株中，不同发育生物膜对链激酶、蛋白酶和过碘酸钠的反应有显著差异（p<0.05）。关于甲氧西林敏感金黄色葡萄球菌分离株，仅对链激酶的不同发育生物膜表现出明显的不同的易感性（p<0.05）。Cis-2-癸烯酸的平均生物膜抑制率为 54.27±27.93%，平均生物膜分散率为 71.92±11.59%，而金缕梅单宁的相应值分别为 83.03±13.95%和 70.48±7.116%，针对新描述的耐甲氧西林金黄色葡萄球菌生物膜表型。
建议对葡萄球菌生物膜和抗生物膜药物应用更具生物学相关性的培养条件。
