Department of Physiology, Istanbul University, Istanbul Faculty of Medicine, Capa, Istanbul, Turkey.
Istanbul University, Institute of Graduate Studies of Health Sciences, Istanbul, Turkey.
Clin Hemorheol Microcirc. 2023;85(1):1-12. doi: 10.3233/CH-221452.
Thalassemia patients have reduced red cell deformability and decreased plasma zinc levels in their blood.
This study aimed to evaluate the effects of zinc (Zn) on the hemorheological parameters and antioxidant enzyme activities in β-thalassemia major (TM) and healthy volunteers (HV).
Hemorheological parameters were measured using LORCA (laser-assisted optical rotational cell analyzer) after adjusting the hematocrit to 40%. Zinc sulfate (ZnSO4.7H2O) was used for Zn incubation with a concentration of 0.5μg/dl. Oxidative stress and antioxidant status were determined using commercial kits.
Data showed that after Zn incubation, EImax, the area under the EI-osmolarity curve (Area), and Omax decreased in TM. However, no significant difference was observed in the osmotic deformability parameters of HV. The increased elongation index was obtained at different shear stresses for TM and HV, and SS1/2 decreased in both groups. The AMP and aggregation index (AI) decreased in TM, and the required time for half of the maximum aggregation (t1/2) increased in HV. However, Zn did not affect oxidative parameters in both groups.
This study showed that Zn incubation increased deformability and decreased aggregation in thalassemic erythrocytes. It means that Zn supplementation will contribute to microcirculation in thalassemia patients.
地中海贫血患者的红细胞变形能力降低，血液中的血浆锌水平降低。
本研究旨在评估锌（Zn）对重型β地中海贫血（TM）和健康志愿者（HV）的血液流变学参数和抗氧化酶活性的影响。
使用 LORCA（激光辅助光学旋转细胞分析仪）在调整血细胞比容至 40%后测量血液流变学参数。使用浓度为 0.5μg/dl 的硫酸锌（ZnSO4.7H2O）进行 Zn 孵育。使用商业试剂盒测定氧化应激和抗氧化状态。
数据显示，Zn 孵育后，TM 的 EImax、EI-渗透压曲线下面积（Area）和 Omax 降低。然而，HV 的渗透变形参数没有显著差异。TM 和 HV 在不同的剪切应力下获得了增加的伸长指数，并且两组的 SS1/2 均降低。AMP 和聚集指数（AI）在 TM 中降低，HV 中达到最大聚集一半所需的时间（t1/2）增加。然而，Zn 对两组的氧化参数均没有影响。
本研究表明，Zn 孵育增加了地中海贫血患者红细胞的变形能力并降低了其聚集性。这意味着 Zn 补充将有助于地中海贫血患者的微循环。
