This present study involves the formation and investigation of the characteristics of a fabricated mat from a PVA-betel leaf mixture. Under ideal processing parameters, nanofibrous mat is synthesized from the PVA-betel leaf blended solution by using the electrospinning technique. Afterwards, the produced nanofibrous mat is assessed for its thermal, antibacterial, morphological, moisture management and chemical interaction behavior using thermogravimetric analysis (TGA), antibacterial assay, scanning electron microscope (SEM), moisture management tester (MMT) and Fourier-transform infrared spectroscopy (FTIR) respectively. The antibacterial action against and bacteria has been assessed using the agar diffusion technique, which reveals the creation of zones of inhibition with a value of about 20 mm. Besides, the fabricated nanomat reveals an average diameter of 183.4 nm with improved moisture and thermal characteristics. Furthermore, the generated nanofibrous mat has all the necessary components, as evidenced by the distinctive peaks in the FTIR spectra. Hence, the recently developed nanofibrous mat exhibits promising potential as a suitable material for wound dressing applications.