Molapour Rashedi Samira, Khajavi Ramin, Rashidi Abosaeed, Rahimi Mohammad Karim, Bahador Abbas
Department of Textile Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Polymer and Textile Engineering, South Tehran Branch, Islamic Azad University, Tehran, Iran.
Iran J Biotechnol. 2021 Jul 1;19(3):e2737. doi: 10.30498/ijb.2021.220458.2737. eCollection 2021 Jul.
Chronic wounds contribute to the majority of clinical cases, associated with significant morbidity, and place a massive financial burden on healthcare systems. Thus, various bandage mats have been designed to facilitate wound healing in clinical applications. Polylactic acid (PLA) nanofibers, as suitable drug carriers, are highly desirable to prepare a controlled environment for wound healing in dressing tissue. Zinc oxide (ZnO) nanoparticles as an effective antibacterial agent for wound treatment prevent bacterial invasion and wound infection.
In this project, for the first time, a new (PLA)/(ZnO) nanofibrous nanocomposite loaded with tranexamic acid (TXA) has been introduced as a useable dressing in wound healing. Furthermore, the antibacterial properties, coagulant assay, and wound healing assays of nanocomposite are evaluated.
PLA/ZnO nanofibrous nanocomposites were loaded with tranexamic acid fabricated by electrospinning method at distinct concentrations. The prepared structure was characterized using field emission scanning electron microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDS), and Fourier transform infrared spectroscopy (FTIR). Further, antimicrobial properties of tissue were investigated against and bacteria. Also, the coagulation assays, cytotoxicity, and skin wound healing model in mice were evaluated.
Morphological analysis of the prepared nanofibrous nanocomposites showed uniform bead-free nanofibers with an average size of 90 nm diameter. The structure exhibited proper antibacterial activities against and bacteria, and a good blood clotting effect. cytotoxicity assay of the structure approved that this mat has no cytotoxic effect on human dermal fibroblast cells. wound healing examination in mice observed over 7 and 14 days showed a faster rate of wound healing over the control.
Novel electrospun PLA/ZnO nanocomposites loaded with tranexamic acid can be prepared by the electrospinning method and used for wound treatment. This structure displayed the effect of two agents in wound healing, including antibacterial nanoparticles and antifibrinolytic drugs to accelerate wound closure.
慢性伤口占大多数临床病例，伴有显著的发病率，并给医疗系统带来巨大的经济负担。因此，人们设计了各种绷带垫以促进临床应用中的伤口愈合。聚乳酸（PLA）纳米纤维作为合适的药物载体，非常适合在敷料组织中为伤口愈合营造可控环境。氧化锌（ZnO）纳米颗粒作为伤口治疗的有效抗菌剂，可防止细菌入侵和伤口感染。
在本项目中，首次引入了一种负载氨甲环酸（TXA）的新型（PLA）/（ZnO）纳米纤维纳米复合材料作为伤口愈合的可用敷料。此外，还评估了该纳米复合材料的抗菌性能、凝血试验和伤口愈合试验。
通过静电纺丝法制备了不同浓度负载氨甲环酸的PLA/ZnO纳米纤维纳米复合材料。使用场发射扫描电子显微镜（FESEM）、能量色散X射线光谱（EDS）和傅里叶变换红外光谱（FTIR）对制备的结构进行表征。此外，研究了该组织对金黄色葡萄球菌和大肠杆菌的抗菌性能。还评估了凝血试验、细胞毒性以及小鼠皮肤伤口愈合模型。
对制备的纳米纤维纳米复合材料的形态分析表明，其具有均匀的无珠纳米纤维，平均直径为90nm。该结构对金黄色葡萄球菌和大肠杆菌表现出良好的抗菌活性以及良好的凝血效果。该结构的细胞毒性试验证实，这种垫子对人皮肤成纤维细胞没有细胞毒性作用。在小鼠身上进行的7天和14天伤口愈合检查显示，与对照组相比，伤口愈合速度更快。
通过静电纺丝法可制备负载氨甲环酸的新型电纺PLA/ZnO纳米复合材料，并用于伤口治疗。这种结构在伤口愈合中显示了两种药剂的作用，包括抗菌纳米颗粒和抗纤维蛋白溶解药物，以加速伤口闭合。
