Choi Sang-Pil, Park Si-Won, Kang Seok-Jin, Lim Seul Ki, Kwon Min-Sung, Choi Hak-Jong, Chun Taehoon
Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea.
Technology Innovation Research Division, World Institute of Kimchi, Gwangju 61755, Korea.
Food Sci Anim Resour. 2023 Jul;43(4):703-711. doi: 10.5851/kosfa.2023.e23. Epub 2023 Jul 1.
As an initial study to elucidate the molecular mechanism of how probiotics modulate macrophage activity, we monitored mRNA expression patterns in peritoneal macrophages (PMs) treated with two different strains of probiotics. After treatment with either WIKIM28 or WIKIM50, total RNAs from PMs were isolated and subjected into gene chip analyses. As controls, mRNAs from vehicle (phosphate-buffered saline, PBS)-treated PMs were also subjected to gene chip analysis. Compared to vehicle (PBS)-treated PMs, WIKIM28-treated and WIKIM50-treated PMs exhibited a total of 889 and 432 differentially expressed genes with expression differences of at least 4 folds, respectively. Compared to WIKIM28-treated PMs, WIKIM50-treated PMs showed 25 up-regulated genes and 21 down-regulated genes with expression differences of more than 2 folds. Interestingly, mRNA transcripts of M2 macrophage polarization marker such as , , and were increased in WIKIM50-treated PMs comparing to those in WIKIM28-treated PMs. Reversely, mRNA transcripts of M1 macrophage polarization marker such as , , and were decreased in WIKIM50-treated PMs comparing to those in WIKIM28-treated PMs. In agreement with these observations, mRNA expression levels of tumor necrosis factor-α and interleukin-1α were significantly reduced in WIKIM50-treated macrophages compared to those in WIKIM28-treated macrophages. These results may indicate that probiotics can be classified as two different types depending on their ability to convert macrophages into M1 or M2 polarization.
作为阐明益生菌调节巨噬细胞活性分子机制的初步研究，我们监测了用两种不同益生菌菌株处理的腹膜巨噬细胞（PMs）中的mRNA表达模式。在用WIKIM28或WIKIM50处理后，分离出PMs的总RNA并进行基因芯片分析。作为对照，来自载体（磷酸盐缓冲盐水，PBS）处理的PMs的mRNA也进行了基因芯片分析。与载体（PBS）处理的PMs相比，WIKIM28处理和WIKIM50处理的PMs分别显示共有889个和432个差异表达基因，表达差异至少为4倍。与WIKIM28处理的PMs相比，WIKIM50处理的PMs显示25个上调基因和21个下调基因，表达差异超过2倍。有趣的是，与WIKIM28处理的PMs相比，WIKIM50处理的PMs中M2巨噬细胞极化标志物如、和的mRNA转录物增加。相反，与WIKIM28处理的PMs相比，WIKIM50处理的PMs中M1巨噬细胞极化标志物如、和的mRNA转录物减少。与这些观察结果一致，与WIKIM28处理的巨噬细胞相比，WIKIM50处理的巨噬细胞中肿瘤坏死因子-α和白细胞介素-1α的mRNA表达水平显著降低。这些结果可能表明，益生菌可根据其将巨噬细胞转化为M1或M2极化的能力分为两种不同类型。
