Wei Wei, Wei Xuan, Xie Xin
Department of Cardiothoracic Surgery, Zhuhai People's Hospital, Zhuhai Hospital Affiliated with Jinan University, Zhuhai 519000, China.
Department of Radiation Oncology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510520, China.
Iran J Public Health. 2023 Jun;52(6):1170-1180. doi: 10.18502/ijph.v52i6.12959.
Esophageal squamous cell carcinoma (ESCC), a widely known esophageal disease, severely affects people's health. Numerous investigations demonstrated that long non-coding RNAs (lncRNAs) performed key jobs inside a wide scope of organic cycles and stand out in malignant growth. Our study planned to investigate the roles and mechanisms of linc01614 in ESCC.
A Total of 60 ESCC tissue samples including 30 patients with cisplatin sensitivity and 30 patients with cisplatin resistance, who received DDP-based treatment, were obtained from Zhuhai People's Hospital, Zhuhai City during 2021. These tissues were frozen and saved in a -80 °C ultra-low temperature freezer. We performed CCK-8, clone formation, flow cytometry assays to determine the effect of linc01614 on ESCC progression, and explored the specific mechanism of linc01614 in ESCC cell proliferation, apoptosis, and chemotherapy resistance.
linc01614 expression was upregulated in ESCC tissues and cells compared with non-tumor tissues and human normal esophageal epithelial cells (Het-1A). Knockdown of linc01614 repressed cell expansion, chemotherapy opposition, and advanced cell apoptosis in ESCC. Besides, linc01614 regulated the expression of miR-4775 as a competitive endogenous RNA (ceRNA).
The linc01614/miR-4775 axis played an important role in ESCC progression and drug resistance, revealing that linc01614 is a promising target in ESCC treatment.
食管鳞状细胞癌（ESCC）是一种广为人知的食管疾病，严重影响人们的健康。大量研究表明，长链非编码RNA（lncRNAs）在广泛的生物过程中发挥关键作用，在肿瘤中备受关注。我们的研究旨在探讨linc01614在ESCC中的作用及机制。
2021年期间，从珠海市人民医院获取了60例ESCC组织样本，其中包括30例对顺铂敏感和30例对顺铂耐药且接受基于顺铂治疗的患者。这些组织被冷冻并保存在-80°C超低温冰箱中。我们进行了CCK-8、克隆形成、流式细胞术检测，以确定linc01614对ESCC进展的影响，并探讨linc01614在ESCC细胞增殖、凋亡和化疗耐药中的具体机制。
与非肿瘤组织和人正常食管上皮细胞（Het-1A）相比，linc01614在ESCC组织和细胞中表达上调。敲低linc01614可抑制ESCC细胞的增殖、化疗耐药性并促进细胞凋亡。此外，linc01614作为竞争性内源性RNA（ceRNA）调节miR-4775的表达。
linc01614/miR-4775轴在ESCC进展和耐药中起重要作用，表明linc01614是ESCC治疗中一个有前景的靶点。
