Powell J A, Rieger F, Holmes N
Neurosci Lett. 1986 Aug 4;68(3):277-81. doi: 10.1016/0304-3940(86)90502-1.
Dysgenic (mdg/mdg) skeletal muscle of the mouse, grown in primary cell culture, fires action potentials in the absence of contractions, thus allowing analysis of the role of electrical activity (without contraction) on the specific activity and molecular forms of acetylcholinesterase (AChE). Specific activity of AChE was assessed by the spectrophometric method of Ellman (Biochem. Pharmacol., 7 (1961) 88-95) and found to increase by 2-5 times in the active myotubes (contraction and action potentials in normal and action potentials, alone in dysgenic muscle) compared to quiescent muscle. Sucrose density sedimentation analysis of muscle homogenates revealed an increase, by 2-3 times, in the proportion of the asymmetric (16S) molecular form of AChE in active muscle of both genotypes. Thus, electrical membrane activity, and not contraction per se, is directly involved in the regulation of levels of specific activity of and accumulation of the asymmetric (16S) form of AChE in muscle cells in culture.
在原代细胞培养中生长的小鼠发育异常（mdg/mdg）骨骼肌，在没有收缩的情况下产生动作电位，从而能够分析电活动（无收缩）对乙酰胆碱酯酶（AChE）比活性和分子形式的作用。采用Ellman的分光光度法（《生物化学与药理学》，7 (1961) 88 - 95）评估AChE的比活性，发现与静止肌肉相比，活跃肌管（正常情况下有收缩和动作电位，发育异常的肌肉中仅有动作电位）中的AChE比活性增加了2至5倍。对肌肉匀浆进行蔗糖密度沉降分析发现，两种基因型的活跃肌肉中，AChE不对称（16S）分子形式的比例增加了2至3倍。因此，电膜活动而非收缩本身，直接参与调节培养的肌肉细胞中AChE比活性水平以及不对称（16S）形式的积累。
