Porcino Gabriane Nascimento, Bladergroen Marco René, Dotz Viktoria, Nicolardi Simone, Memarian Elham, Gardinassi Luiz Gustavo, Nery Costa Carlos Henrique, Pacheco de Almeida Roque, Ferreira de Miranda Santos Isabel Kinney, Wuhrer Manfred
Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto 14049-900, Brazil.
Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands.
iScience. 2023 Jun 5;26(7):107021. doi: 10.1016/j.isci.2023.107021. eCollection 2023 Jul 21.
Visceral leishmaniasis (VL) is a clinical form of leishmaniasis with high mortality rates when not treated. Diagnosis suffers from invasive techniques and sub-optimal sensitivities. The current (affordable) treatment with pentavalent antimony as advised by the WHO is possibly harmful to the patient. There is need for an improved diagnosis to prevent possibly unnecessary treatment. -glycan analysis may aid in diagnosis. We evaluated the -glycan profiles from active VL, asymptomatic infections (ASYMP) and controls from non-endemic (NC) and endemic (EC) areas. Active VL has a distinct -glycome profile that associates with disease severity. Our study suggests that the observed glycan signatures could be a valuable additive to diagnosis and assist in identifying possible markers of disease and understanding the pathogenesis of VL. Further studies are warranted to assess a possible future role of blood glycome analysis in active VL diagnosis and should aim at disease specificity.
内脏利什曼病（VL）是利什曼病的一种临床形式，若不治疗，死亡率很高。诊断依赖侵入性技术且敏感性欠佳。世界卫生组织建议的当前（可负担的）五价锑治疗可能对患者有害。需要改进诊断方法以避免可能不必要的治疗。聚糖分析可能有助于诊断。我们评估了来自活跃性VL、无症状感染（ASYMP）以及非流行区（NC）和流行区（EC）对照的聚糖谱。活跃性VL具有与疾病严重程度相关的独特聚糖组谱。我们的研究表明，观察到的聚糖特征可能是诊断的有价值补充，并有助于识别可能的疾病标志物以及理解VL的发病机制。有必要进行进一步研究以评估血液聚糖组分析在活跃性VL诊断中未来可能发挥的作用，且应以疾病特异性为目标。
