Department of Public Health, Xinjiang Medical University, Xinjiang, China.
Drug Clinical Institutions, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
Neurol Res. 2023 Sep;45(9):804-817. doi: 10.1080/01616412.2023.2203616. Epub 2023 Jul 24.
Long non-coding RNAs (lncRNAs) play critical role in the pathogenesis of neurodegenerative diseases. Human plasma contains lncRNAs that are present in the blood, and their disease-specific profile has been considered a potential biomarker in some diseases.
This study reports screening of the plasma levels of lncRNAs between Alzheimer disease(AD) ( = 45) and matched healthy controls ( = 45). The plasma samples of 5 AD patients and 5 matched healthy controls were randomly selected for expression levels of lncRNAs using the TruSeq RNA Sample Prep Kit (Illumina). The receiver operating characteristic (ROC) curve and area under the curve (AUC) were used to study the potential of lncRNAs as biomarkers.
The differential expression profiles of plasma showed that 514 lncRNAs were upregulated, whereas 499 lncRNAs were downregulated.We found that the lncRNAs AL133415.1, AC020916.1, ENST00000654948, ASMTL-AS, AC005730.3, and AP001363.1 levels in the plasma of the AD patients were significantly lower compared to the control group (p1 = 0.0006, p2 < 0.001, p3 < 0.001, p4 = 0.039, p5 = 0.006, p6 < 0.001, respectively). ROC curve analysis revealed that the AUC of AL133415.1 was 0.635 (95% CI]: 0.507-0.763, = 0.036), the AUC of ASMTL-AS1 was 0.658 (95% CI: 0.513-0.785, = 0.015), the AUC of AC005730.3 was 0.627 (95%CI: 0.498-0.756, = 0.049), and the AUC of AP001363.1 was 0.708 (95%CI: 0.595-0.822, = 0.001).
This study indicated that the plasma levels of the lncRNAs ASMTL-AS1, AP001363.1, AC005730.3, and AL133415.1 might be considered potential biomarkers for AD in the Chinese Population.
长非编码 RNA（lncRNA）在神经退行性疾病的发病机制中发挥着关键作用。人类血浆中含有存在于血液中的 lncRNA，其疾病特异性特征已被认为是某些疾病的潜在生物标志物。
本研究报告了在阿尔茨海默病（AD）患者（ = 45）和匹配的健康对照组（ = 45）之间筛选血浆 lncRNA 水平。使用 TruSeq RNA 样品制备试剂盒（Illumina）随机选择 5 名 AD 患者和 5 名匹配的健康对照者的血浆样本，检测 lncRNA 的表达水平。采用受试者工作特征（ROC）曲线和曲线下面积（AUC）研究 lncRNA 作为生物标志物的潜力。
差异表达谱显示，血浆中 514 个 lncRNA 上调，499 个 lncRNA 下调。我们发现 AD 患者血浆中的 lncRNA AL133415.1、AC020916.1、ENST00000654948、ASMTL-AS、AC005730.3 和 AP001363.1 水平明显低于对照组（p1 = 0.0006、p2 < 0.001、p3 < 0.001、p4 = 0.039、p5 = 0.006、p6 < 0.001）。ROC 曲线分析显示，AL133415.1 的 AUC 为 0.635（95%CI：0.507-0.763， = 0.036），ASMTL-AS1 的 AUC 为 0.658（95%CI：0.513-0.785， = 0.015），AC005730.3 的 AUC 为 0.627（95%CI：0.498-0.756， = 0.049），AP001363.1 的 AUC 为 0.708（95%CI：0.595-0.822， = 0.001）。
本研究表明，血浆中 lncRNA ASMTL-AS1、AP001363.1、AC005730.3 和 AL133415.1 的水平可能被认为是中国人群 AD 的潜在生物标志物。
