National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kochi, India.
Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Kochi, India.
Arch Virol. 2023 Jul 24;168(8):211. doi: 10.1007/s00705-023-05827-1.
Infectious hypodermal hematopoietic necrosis virus (IHHNV/PstDVI) was isolated and propagated in the hybrid shrimp-insect cell line PmLyO-Sf9. A few hours after inoculation with an infected tissue extract or virus suspension, cytopathic changes could be observed in the cell line, including clustering, enlargement, syncytium formation, granulation, vacuole formation, tapering, irregularities in the plasma membrane with extended tails, detachment, cell death, and accumulation of cellular debris. Expression of viral genes, the presence of virions, and cytological changes observed using transmission electron microscopy suggested replication of the virus in these cells. The virus was purified by ultracentrifugation, negatively stained, and examined using an electron microscope, and the purified virus was found to be infectious both in vitro and in vivo. This development opens avenues for the study of the basic molecular mechanism of IHHNV infection, pathogenesis, and replication, which is much needed for developing an antiviral strategy in aquaculture.
传染性皮下及造血组织坏死病毒（IHHNV/PstDVI）在杂交虾-昆虫细胞系 PmLyO-Sf9 中分离和增殖。接种感染组织提取物或病毒悬浮液后几个小时，细胞系中可观察到细胞病变，包括聚集、增大、合胞体形成、颗粒化、空泡形成、变细、质膜不规则并带有长尾、脱落、细胞死亡和细胞碎片积累。病毒基因的表达、病毒粒子的存在以及透射电子显微镜观察到的细胞学变化表明病毒在这些细胞中复制。病毒通过超速离心、负染色和电子显微镜检查进行纯化，纯化的病毒在体外和体内均具有感染性。这一发展为研究 IHHNV 感染、发病机制和复制的基本分子机制开辟了途径，这对于在水产养殖中开发抗病毒策略非常必要。
