Antares I: a Modular Photobioreactor Suitable for Photosynthesis and Bioenergetics Research.

Oxygenic photosynthesis is responsible for most of the fixation of atmospheric CO. The microalgal community can transport atmospheric carbon into biological cycles in which no additional CO is created. This represents a resource to confront the actual climate change crisis. These organisms have evolved to adapt to several environments and different spectral distribution of light that may strongly influence their metabolism. Therefore, there is a need for development of photobioreactors specialized in addressing spectral optimization. Here, a multi-scale modular photobioreactor made from standard glass materials, ad hoc light circuits, and easily accessible, small commercial devices is described. The system is suitable to manage the principal culture variables of research in bioenergetics and photosynthesis. Its performance was tested by growing four evolutionary-distant microalgal species with different endosymbiotic scenarios: Chlamydomonas reinhardtii (Archaeplastida, green primary plastid), Polytomella parva (Archaeplastida, colorless plastid), Euglena gracilis (Discoba, green secondary plastid), and Phaeodactylum tricornutum (Stramenophiles, red secondary plastid). Our results show an improvement of biomass production, as compared to the traditional flask system. The modulation of the incident light spectra allowed us to observe a far-red adaptation in Euglena gracilis with a difference on paramylon production, and it also significantly increased the maximal cell density of the diatom species under green light. Together, these confirm that for photobioreactors with artificial light, manipulation of the light spectrum is a critical parameter for controlling the optimal performance, depending on the downstream goals.