Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta T6G 1H9, Canada.
Heavy Machinery Engineering Research Center of Education Ministry, Taiyuan University of Science and Technology, Taiyuan 030024, China.
J Colloid Interface Sci. 2023 Nov 15;650(Pt B):1525-1535. doi: 10.1016/j.jcis.2023.07.108. Epub 2023 Jul 18.
Conventional coating strategies and materials for bio-applications with protective, diagnostic, and therapeutic functions are commonly limited by their arduous preparation processes and lack of on-demand functionalities. Herein, inspired by the 'root-leaf' structure of grass, a series of novel polyacrylate-conjugated proteins can be engineered with sticky bovine serum albumin (BSA) protein as a 'root' anchoring layer and a multifunctional polyacrylate as a 'leaf' functional layer for the facile coating procedure and versatile surface functionalities.
The engineered proteins were synthesized based on click chemistry, where the 'root' layer can universally anchor onto both organic and inorganic substrates through a facile dip/spraying method with excellent stability in harsh solution conditions, thanks to its multiple adaptive molecular interactions with substrates that further elucidated by molecular force measurements between the 'root' BSA protein and substrates. The 'leaf' conjugated-polyacrylates imparted coatings with versatile on-demand functionalities, such as resistance to over 99% biofouling in complex biofluids, pH-responsive performance, and robust adhesion with various nanomaterials.
By synergistically leveraging the universal anchoring capabilities of BSA with the versatile physicochemical properties of polyacrylates, this study introduces a promising and facile strategy for imparting novel functionalities to a myriad of surfaces through engineering natural proteins and biomaterials for biotechnical and nanotechnical applications.
具有保护、诊断和治疗功能的生物应用的常规涂层策略和材料通常受到其繁琐的制备过程和缺乏按需功能的限制。受草“根-叶”结构的启发，本文设计了一系列新型的聚丙烯酸酯偶联蛋白，其中粘性牛血清白蛋白（BSA）蛋白作为“根”锚固层，多功能聚丙烯酸酯作为“叶”功能层，用于简便的涂层程序和多功能表面功能。
根据点击化学原理合成了工程蛋白，其中“根”层可以通过简便的浸渍/喷涂方法普遍锚定在有机和无机基底上，并且在恶劣的溶液条件下具有优异的稳定性，这要归功于其与基底的多种适应性分子相互作用，这些相互作用通过“根”BSA 蛋白与基底之间的分子力测量进一步阐明。偶联的“叶”聚丙烯酸酯赋予涂层多种按需功能，例如在复杂的生物流体中抵抗超过 99%的生物污垢、pH 响应性能以及与各种纳米材料的牢固粘附。
本研究通过协同利用 BSA 的通用锚固能力和聚丙烯酸酯的多功能物理化学性质，为通过工程天然蛋白质和生物材料为生物技术和纳米技术应用赋予各种表面新功能引入了一种有前途且简便的策略。
