Cheng Liang, Zhang Song, Zhang Qian, Gao Wenjie, Wang Benfeng, Mu Shengzhi
Department of Burns and Plastic Surgery, Shaanxi Provincial People's Hospital, Xi'an, 710068, China.
Heliyon. 2024 Jun 15;10(12):e32864. doi: 10.1016/j.heliyon.2024.e32864. eCollection 2024 Jun 30.
Hydrogels exhibit exceptional suitability as wound dressing due to their remarkable three-dimensional (3D) characteristics. Here, we have reported the fabrication of hydrogels from biopolymers carboxymethyl cellulose (CMC), polyvinyl alcohol (PVA), and gelatin via a simple blending method to mimic the natural extracellular matrix. Scanning electron microscopy (SEM), water contact meters (WCM), and Fourier-transform infrared spectroscopy (FTIR) were used to evaluate the chemical structural, morphological, and wettability behavior. The wetting and degradation behavior were also found to be different for different formulations (Min. (51.60) and Max. (113.60)) and (Min. (38.82 mg) and Max. (3.72 mg)), respectively. Swelling was investigated in different media, including phosphate buffer saline solution (PBS) and aqueous media. It was observed that the hydrogel displayed the highest degree of swelling in an aqueous medium (Min. (597.32-1121.49 %) and Max. (1089.51-2139.73 %)) compared to PBS media (Min. (567.01-1021.85 %) and Max. (899.13-1639.17 %)). The release of Neomycin was studied in a PBS medium via the Franz diffusion method at 37 °C. The maximal release in various media demonstrated pH-responsive behavior. The viability and proliferation of fibroblast (3T3) cell lines were examined in vitro to evaluate cytocompatibility. Human Embryonic Kidney (HEK) 293 cells were used to evaluate the hydrogels' ability to promote vascularization and angiogenesis. Therefore, the data demonstrate that hydrogels that have been manufactured have qualities that make them promising for use as wound dressings in wound healing applications.
水凝胶因其卓越的三维（3D）特性而表现出作为伤口敷料的非凡适用性。在此，我们报道了通过一种简单的共混方法，由生物聚合物羧甲基纤维素（CMC）、聚乙烯醇（PVA）和明胶制备水凝胶，以模拟天然细胞外基质。使用扫描电子显微镜（SEM）、水接触仪（WCM）和傅里叶变换红外光谱（FTIR）来评估化学结构、形态和润湿性行为。还发现不同配方的润湿和降解行为有所不同（分别为最小值（51.60）和最大值（113.60））以及（最小值（38.82毫克）和最大值（3.72毫克））。在包括磷酸盐缓冲盐水溶液（PBS）和水性介质在内的不同介质中研究了溶胀情况。观察到与PBS介质（最小值（567.01 - 1021.85%）和最大值（899.13 - 1639.17%））相比，水凝胶在水性介质中表现出最高程度的溶胀（最小值（597.32 - 1121.49%）和最大值（1089.51 - 2139.73%））。通过Franz扩散法在37°C下于PBS介质中研究了新霉素的释放。在各种介质中的最大释放表现出pH响应行为。在体外检查了成纤维细胞（3T3）细胞系的活力和增殖，以评估细胞相容性。使用人胚肾（HEK）293细胞来评估水凝胶促进血管形成和血管生成的能力。因此，数据表明所制备的水凝胶具有使其有望在伤口愈合应用中用作伤口敷料的特性。
