College of Life Science, Liaoning Normal University, Dalian, 116081, China; Lamprey Research Center, Liaoning Normal University, Dalian, 116081, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian, 116034, China.
College of Life Science, Liaoning Normal University, Dalian, 116081, China; Lamprey Research Center, Liaoning Normal University, Dalian, 116081, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian, 116034, China.
Fish Shellfish Immunol. 2023 Sep;140:108967. doi: 10.1016/j.fsi.2023.108967. Epub 2023 Jul 23.
The tryptophan-kynurenine (TRP-KYN) pathway is involved in several biological functions, including immunosuppression, inflammatory response, and tumor suppression. Six TRP-KYN pathway-related genes, tryptophan 2,3-dioxygenase (TDO), indoleamine 2,3-dioxygenase 2 (IDO2), aminoadipate aminotransferase (AADAT), glutamate oxaloacetate transaminase 2 (GOT2), kynurenine monooxygenase (KMO), and kynureninase (KYNU) have been identified and cloned from the jawless vertebrate lamprey (Lampetra japonica) to gain insights into their evolution and characterization. Expression distribution showed that the key gene Lj-TDO was highly expressed in the oral gland. Real-time quantitative PCR showed that TRP-KYN pathway-related genes were significantly overexpressed after multi-stimulation. RNA interference showed that Lj-IDO2 knockdown regulated the expression of inflammatory factors. In conclusion, our study successfully clarified the ancestral features and functions of the TRP-KYN pathway, while providing valuable insights into the involvement of this pathway in the immune responses of a jawless vertebrate.
色氨酸-犬尿氨酸（TRP-KYN）途径参与多种生物学功能，包括免疫抑制、炎症反应和肿瘤抑制。已经从无颌脊椎动物七鳃鳗（Lampetra japonica）中鉴定和克隆了六个 TRP-KYN 途径相关基因，包括色氨酸 2,3-双加氧酶（TDO）、吲哚胺 2,3-双加氧酶 2（IDO2）、天冬氨酸氨基转移酶（AADAT）、谷氨酸草酰乙酸转氨酶 2（GOT2）、犬尿氨酸单加氧酶（KMO）和犬尿氨酸酶（KYNU），以深入了解它们的进化和特征。表达分布表明关键基因 Lj-TDO 在口腔腺中高度表达。实时定量 PCR 显示，TRP-KYN 途径相关基因在多刺激后显著过表达。RNA 干扰显示 Lj-IDO2 敲低调节了炎症因子的表达。总之，我们的研究成功阐明了 TRP-KYN 途径的祖征特征和功能，同时为该途径参与无颌脊椎动物的免疫反应提供了有价值的见解。
