Vargas-Chacoff Luis, Nualart Daniela, Vargas-Lagos Carolina, Dann Francisco, Muñoz José Luis, Pontigo Juan Pablo
Laboratorio de Fisiología de Peces, Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia 5090000, Chile.
Centro FONDAP de Investigación en Dinámica de Ecosistemas Marinos de Altas Latitudes (IDEAL), Universidad Austral de Chile, Valdivia 5090000, Chile.
Animals (Basel). 2023 Nov 18;13(22):3562. doi: 10.3390/ani13223562.
Aquaculture fish are kept for long periods in sea cages or tanks. Consequently, accumulated stress causes the fish to present serious problems with critical economic losses. Fish food has been supplemented to reduce this stress, using many components as amino acids such as tryptophan. This study aims to determine the transcriptional effect of tryptophan and cortisol on primary cell cultures of salmon head and posterior kidney. Our results indicate activation of the kynurenine pathway and serotonin activity when stimulated with tryptophan and cortisol. An amount of 95% of tryptophan is degraded by the kynurenine pathway, indicating the relevance of knowing how this pathway is activated and if stress levels associated with fish culture trigger its activation. Additionally, it is essential to know the consequence of increasing kynurenic acid "KYNA" levels in the short and long term, and even during the fish ontogeny.
水产养殖的鱼类长时间饲养在海水网箱或水箱中。因此，累积的应激会导致鱼类出现严重问题，并造成重大经济损失。人们已通过添加鱼类食物来减轻这种应激，使用了许多成分，如色氨酸等氨基酸。本研究旨在确定色氨酸和皮质醇对鲑鱼头肾和后肾原代细胞培养物的转录影响。我们的结果表明，在用色氨酸和皮质醇刺激时，犬尿氨酸途径和血清素活性会被激活。95%的色氨酸会通过犬尿氨酸途径降解，这表明了解该途径如何被激活以及鱼类养殖相关的应激水平是否会触发其激活具有重要意义。此外，了解短期内、长期内甚至在鱼类个体发育过程中犬尿酸“KYNA”水平升高的后果也至关重要。
