柑橘木虱（半翅目：木虱科）成虫取食九里香和沙糖桔后肠道基因表达和细菌群落的比较。

The comparison of gut gene expression and bacterial community in Diaphorina citri (Hemiptera: Liviidae) adults fed on Murraya exotica and 'Shatangju' mandarin (Citrus reticulate cv. Shatangju).

机构信息

Guangzhou City Key Laboratory of Subtropical Fruit Trees Outbreak Control, Institute for Management of Invasive Alien Species, Zhongkai University of Agriculture and Engineering, 313 Yingdong Teaching Building, Guangzhou, 510225, China.

出版信息

BMC Genomics. 2023 Jul 24;24(1):416. doi: 10.1186/s12864-023-09308-2.

DOI:10.1186/s12864-023-09308-2

PMID:37488494

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10364414/

Abstract

BACKGROUND

Diaphorina citri Kuwayama is an important citrus pest. It serves as the vector for the transmission of Candidatus Liberibacter asiaticus (CLas), which induced a destructive disease, Huanglongbing, and caused huge economic losses. During the interaction between insects and plants, insects have evolved a series of mechanisms to adapt to various host plants. Murraya exotica and 'Shatangju' mandarin (Citrus reticulate cv. Shatangju) are the Rutaceae species from different genera that have been discovered as suitable hosts for D. citri adults. While the adaptation mechanism of this pest to these two host plants is unclear.

RESULTS

In this study, RNA-seq and 16 S rDNA amplification sequencing were performed on the gut of D. citri adults reared on M. exotica and 'Shatangju' mandarin. RNA-seq results showed that a total of 964 differentially expressed genes were found in different gut groups with two host plant treatments. The impacted genes include those that encode ribosomal proteins, cathepsins, and mitochondrial respiratory chain complexes. According to 16 S rDNA sequencing, the compositions of the gut bacterial communities were altered by different treatments. The α and β diversity analyses confirmed that the host plant changes influenced the gut microbial diversity. The functional classification analysis by Tax4Fun revealed that 27 KEGG pathways, mostly those related to metabolism, including those for nucleotide metabolism, energy metabolism, metabolism of cofactors and vitamins, amino acid metabolism, carbohydrate metabolism, xenbiotics biodegradation and metabolism, lipid metabolism, and biosynthesis of other secondary metabolites, were significantly altered.

CONCLUSION

Our preliminary findings shed light on the connection between D. citri and host plants by showing that host plants alter the gene expression profiles and bacterial community composition of D. citri adults.

摘要

背景

柑橘木虱 Kuwayama 是一种重要的柑橘害虫。它是 Candidatus Liberibacter asiaticus（CLas）的传播媒介，CLas 引起了破坏性疾病黄龙病，并造成了巨大的经济损失。在昆虫与植物的相互作用中，昆虫进化出了一系列适应各种宿主植物的机制。九里香和沙糖桔（Citrus reticulate cv. Shatangju）是不同属的芸香科植物，已被发现是柑橘木虱成虫的适宜宿主。然而，这种害虫对这两种宿主植物的适应机制尚不清楚。

结果

本研究对在九里香和沙糖桔上饲养的柑橘木虱成虫的肠道进行了 RNA-seq 和 16S rDNA 扩增测序。RNA-seq 结果表明，在两个宿主植物处理的不同肠道组中，共发现了 964 个差异表达基因。受影响的基因包括编码核糖体蛋白、组织蛋白酶和线粒体呼吸链复合物的基因。根据 16S rDNA 测序结果，不同处理改变了肠道细菌群落的组成。α 和 β 多样性分析证实，宿主植物的变化影响了肠道微生物的多样性。Tax4Fun 的功能分类分析显示，27 条 KEGG 途径，主要与代谢有关，包括核苷酸代谢、能量代谢、辅酶和维生素代谢、氨基酸代谢、碳水化合物代谢、外源生物降解和代谢、脂质代谢以及其他次生代谢物的生物合成途径，均发生了显著改变。