Liu Kai, He Jiawei, Guan Ziying, Zhong Mingzhao, Pang Rui, Han Qunxin
College of Agriculture and Biology, Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China.
Front Physiol. 2021 Feb 24;11:630037. doi: 10.3389/fphys.2020.630037. eCollection 2020.
The Asian citrus psyllid is the transmission vector of Huanglongbing (HLB), a devastating disease of citrus plants. The bacterium " Liberibacter asiaticus" (Las) associated with HLB is transmitted between host plants by in a circulative manner. Understanding the interaction between Las and its insect vector is key for protecting citrus cultivation from HLB damage. Here, we used RNA sequencing and liquid chromatography-mass spectrometry (LC-MS) to analyze the transcriptome and metabolome of interacting with Las. We identified 662 upregulated and 532 downregulated genes in Las-infected insects. These genes were enriched in pathways involving carbohydrate metabolism, the insects' immune system, and metabolism of cofactors and vitamins. We also detected 105 differential metabolites between Las-infected and non-infected insects, including multiple nucleosides and lipid-related molecules. The integrated analysis revealed nine pathways-including those of the glycine, serine, threonine, and purine metabolism-affected by the differentially expressed genes from both groups. The network for these pathways was subsequently constructed. Our results thus provide insights regarding the cross-talk between the transcriptomic and metabolomic changes in in response to Las infection, as well as information on the pathways and genes/metabolites related to the Las- interaction.
亚洲柑橘木虱是柑橘黄龙病（HLB）的传播媒介，柑橘黄龙病是一种对柑橘类植物具有毁灭性的病害。与HLB相关的“亚洲韧皮杆菌”（Las）通过循环方式在寄主植物之间传播。了解Las与其昆虫传播媒介之间的相互作用是保护柑橘种植免受HLB危害的关键。在此，我们使用RNA测序和液相色谱-质谱联用（LC-MS）技术分析了与Las相互作用的亚洲柑橘木虱的转录组和代谢组。我们在感染Las的昆虫中鉴定出662个上调基因和532个下调基因。这些基因富集于涉及碳水化合物代谢、昆虫免疫系统以及辅因子和维生素代谢的途径中。我们还检测到感染Las和未感染Las的昆虫之间有105种差异代谢产物，包括多种核苷和脂质相关分子。综合分析揭示了九条受两组差异表达基因影响的途径，包括甘氨酸、丝氨酸、苏氨酸和嘌呤代谢途径。随后构建了这些途径的网络。因此，我们的研究结果为亚洲柑橘木虱响应Las感染时转录组和代谢组变化之间的相互作用提供了见解，同时也提供了与Las-亚洲柑橘木虱相互作用相关的途径以及基因/代谢产物的信息。
