Department of Biology, Institute of Graduate Studies in Science and Engineering, Istanbul University, Istanbul, Turkey.
Department of Biology, Istanbul University, Istanbul, Turkey.
Toxicol Mech Methods. 2023 Nov;33(9):796-805. doi: 10.1080/15376516.2023.2240884. Epub 2023 Aug 1.
Furan is an organic chemical that can cause adverse effects on human health and is formed as a result of the thermal decomposition of many food components during cooking, storage, and processing techniques. Studies have shown that exposure to furan causes nephrotoxicity, hepatotoxicity, immunotoxicity, and reproductive toxicity. According to our current knowledge of the literature, the genotoxic mode of action of furan is highly controversial. The genotoxic effects of furan on the male reproductive system, however, have not been studied. In this study, the TM3 Leydig cell line was treated with 750, 1500, and 3000 μM concentrations of furan for 24 h. Following the completion of the exposure period, the cytotoxicity of furan in TM3 Leydig cells was assessed using a cell viability assay and a spectrophotometric measurement of lactate dehydrogenase (LDH) enzyme levels. The double fluorescence staining method was used to demonstrate furan-induced apoptosis, and DNA damage was shown using the micronucleus, comet, and chromosomal aberration assays. The result indicated that furan administration of Leydig cells resulted in an increase in structural chromosomal aberration, comet, and micronucleus formation, reduced cell viability, increased LDH activity, and a higher incidence of apoptotic cells. These findings revealed that furan induces DNA damage in TM3 Leydig cells, causing genotoxicity and DNA damage-induced cytotoxicity.
呋喃是一种有机化合物，可对人类健康造成不良影响，它是在烹饪、储存和加工过程中，许多食物成分热分解而形成的。研究表明，接触呋喃会导致肾毒性、肝毒性、免疫毒性和生殖毒性。根据我们目前对文献的了解，呋喃的遗传毒性作用模式存在很大争议。然而，呋喃对男性生殖系统的遗传毒性作用尚未得到研究。在这项研究中，TM3 间质细胞瘤系用 750、1500 和 3000μM 浓度的呋喃处理 24 小时。暴露期结束后，通过细胞活力测定和分光光度法测定乳酸脱氢酶（LDH）酶水平来评估 TM3 间质细胞瘤中呋喃的细胞毒性。采用双荧光染色法显示呋喃诱导的细胞凋亡，并用微核、彗星和染色体畸变试验显示 DNA 损伤。结果表明，呋喃处理间质细胞瘤导致结构染色体畸变、彗星和微核形成增加，细胞活力降低，LDH 活性增加，凋亡细胞比例增加。这些发现表明，呋喃诱导 TM3 间质细胞瘤的 DNA 损伤，导致遗传毒性和 DNA 损伤诱导的细胞毒性。
