Aydin Yasemin, Yilmaz Buse, Dikbasan Yasemin U, Orta-Yilmaz Banu
Department of Biology, Faculty of Science, Istanbul University, Istanbul 34116, Turkey.
Institute of Graduate Studies in Science and Engineering, Department of Biology, Istanbul University, Istanbul 34116, Turkey.
Toxicol Res (Camb). 2023 Apr 20;12(3):400-407. doi: 10.1093/toxres/tfad025. eCollection 2023 Jun.
Research on heat-induced food contamination is being given more attention as a result of the health risks that have been publicly revealed in recent years. Furan is known as a colorless, combustible, heterocyclic aromatic organic molecule and is formed when food products are processed and stored. It has been established that furan, which is inevitably ingested, has a deleterious impact on human health and causes toxicity. Furan is known to have adverse effects on the immune system, neurological system, skin, liver, kidney, and fat tissue. Infertility caused by furan is a result of its damaging effects on several tissues and organs as well as the reproductive system. Although studies on the adverse effects of furan on the male reproductive system have been performed, there is no study revealing apoptosis in Leydig cells at the gene level. In this study, TM3 mouse Leydig cells were exposed to 250- and 2,500-μM concentrations of furan for 24 h. The findings demonstrated that furan decreased cell viability and antioxidant enzyme activity while increasing lipid peroxidation, reactive oxygen species, and apoptotic cell rates. Furan also increased the expression of the important apoptotic genes and while decreasing the expression of another pro-apoptotic gene, , and antioxidant genes , , and . In conclusion, these results imply that furan may cause loss of cell function in mouse Leydig cells responsible for testosterone biosynthesis by impairing the efficiency of the antioxidant system, possibly by inducing cytotoxicity, oxidative stress, and apoptosis.
近年来，由于已公开揭示的健康风险，热致食品污染的研究受到了更多关注。呋喃是一种无色、可燃的杂环芳香有机分子，在食品加工和储存过程中形成。已经确定，不可避免摄入的呋喃会对人体健康产生有害影响并导致毒性。已知呋喃会对免疫系统、神经系统、皮肤、肝脏、肾脏和脂肪组织产生不良影响。呋喃导致的不育是其对多个组织和器官以及生殖系统造成损害的结果。虽然已经开展了关于呋喃对雄性生殖系统不良影响的研究，但尚无研究在基因水平揭示睾丸间质细胞中的细胞凋亡情况。在本研究中，将TM3小鼠睾丸间质细胞暴露于浓度为250 μM和2500 μM的呋喃中24小时。研究结果表明，呋喃降低了细胞活力和抗氧化酶活性，同时增加了脂质过氧化、活性氧和凋亡细胞率。呋喃还增加了重要凋亡基因和的表达，同时降低了另一个促凋亡基因以及抗氧化基因、和的表达。总之，这些结果表明，呋喃可能通过损害抗氧化系统的效率，可能是通过诱导细胞毒性、氧化应激和凋亡，导致负责睾酮生物合成的小鼠睾丸间质细胞丧失细胞功能。
