Institute of Physical Chemistry, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, Giessen 35392, Germany.
Center for Materials Research, Justus Liebig University Giessen, Heinrich-Buff-Ring 16, Giessen 35392, Germany.
Biointerphases. 2023 Jul 1;18(4). doi: 10.1116/6.0002820.
The purpose of this Tutorial is to highlight the suitability of time-of-flight secondary ion mass spectrometry (ToF-SIMS) and OrbiTrap™ SIMS (Orbi-SIMS) in bone research by introducing fundamentals and best practices of bone analysis with these mass spectrometric imaging (MSI) techniques. The Tutorial includes sample preparation, determination of best-suited measurement settings, data acquisition, and data evaluation, as well as a brief overview of SIMS applications in bone research in the current literature. SIMS is a powerful analytical technique that allows simultaneous analysis and visualization of mineralized and nonmineralized bone tissue, bone marrow as well as implanted biomaterials, and interfaces between bone and implants. Compared to histological staining, which is the standard analytical procedure in bone research, SIMS provides chemical imaging of nonstained bone sections that offers insights beyond what is conventionally obtained. The Tutorial highlights the versatility of ToF- and Orbi-SIMS in addressing important questions in bone research. By illustrating the value of these MSI techniques, it demonstrates how they can contribute to advance progress in bone research.
本教程旨在通过介绍飞行时间二次离子质谱（ToF-SIMS）和轨道阱 SIMS（Orbi-SIMS）在骨研究中的基本原理和最佳实践，强调它们在骨研究中的适用性。教程包括样品制备、确定最佳测量设置、数据采集和数据评估，以及 SIMS 在当前文献中在骨研究中的应用的简要概述。SIMS 是一种强大的分析技术，允许同时分析和可视化矿化和非矿化骨组织、骨髓以及植入生物材料，以及骨与植入物之间的界面。与组织学染色相比，组织学染色是骨研究中的标准分析程序，SIMS 提供了非染色骨切片的化学成像，提供了超出传统方法获得的见解。本教程强调了 ToF 和 Orbi-SIMS 在解决骨研究中重要问题方面的多功能性。通过说明这些 MSI 技术的价值，它展示了它们如何有助于推进骨研究的进展。
