Noun Manale, Akoumeh Rayane, Abbas Imane
Lebanese Atomic Energy Commission - NCSR, Beirut, Lebanon.
Microsc Microanal. 2022 Feb;28(1):1-26. doi: 10.1017/S1431927621013593.
The potential of mass spectrometry imaging (MSI) has been demonstrated in cell and tissue research since 1970. MSI can reveal the spatial distribution of a wide range of atomic and molecular ions detected from biological sample surfaces, it is a powerful and valuable technique used to monitor and detect diverse chemical and biological compounds, such as drugs, lipids, proteins, and DNA. MSI techniques, notably matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) and time of flight secondary ion mass spectrometry (TOF-SIMS), witnessed a dramatic upsurge in studying and investigating biological samples especially, cells and tissue sections. This advancement is attributed to the submicron lateral resolution, the high sensitivity, the good precision, and the accurate chemical specificity, which make these techniques suitable for decoding and understanding complex mechanisms of certain diseases, as well as monitoring the spatial distribution of specific elements, and compounds. While the application of both techniques for the analysis of cells and tissues is thoroughly discussed, a briefing of MALDI-TOF and TOF-SIMS basis and the adequate sampling before analysis are briefly covered. The importance of MALDI-TOF and TOF-SIMS as diagnostic tools and robust analytical techniques in the medicinal, pharmaceutical, and toxicology fields is highlighted through representative published studies.
自1970年以来，质谱成像（MSI）的潜力已在细胞和组织研究中得到证明。MSI可以揭示从生物样品表面检测到的各种原子和分子离子的空间分布，它是一种用于监测和检测多种化学和生物化合物（如药物、脂质、蛋白质和DNA）的强大而有价值的技术。MSI技术，特别是基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF）和飞行时间二次离子质谱（TOF-SIMS），在研究和调查生物样品（尤其是细胞和组织切片）方面取得了显著进展。这一进展归因于亚微米级的横向分辨率、高灵敏度、良好的精度和准确的化学特异性，这些特性使这些技术适用于解码和理解某些疾病的复杂机制，以及监测特定元素和化合物的空间分布。在全面讨论这两种技术在细胞和组织分析中的应用的同时，还简要介绍了MALDI-TOF和TOF-SIMS的基本原理以及分析前的适当采样。通过代表性的已发表研究，强调了MALDI-TOF和TOF-SIMS作为医学、制药和毒理学领域诊断工具和强大分析技术的重要性。
