State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, PR China.
School of Life Sciences, Jiaying University, Meizhou, 514015, PR China.
Int J Syst Evol Microbiol. 2023 Jul;73(7). doi: 10.1099/ijsem.0.005990.
Two Gram-stain-negative strains, designated as SYSU D00286 and SYSU D00782, were isolated from a sand sample collected from the Kumtag Desert in Xinjiang, north-west China. Cells were aerobic, non-motile and positive for both oxidase and catalase. Growth occurred at 4-37 °C (optimum, 28-30 °C), pH 6.0-7.0 (optimum, pH 7.0) and NaCl concentration of 0-1.5 % (w/v; optimum, 0%). Growth was observed on Reasoner's 2A agar and nutrient agar, but not on Luria-Bertani agar and trypticase soy agar. The polar lipids were identified as diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, three unidentified aminolipids, one unidentified glycolipid and two unidentified phospholipids. The major respiratory quinone was ubiquinone-10 and the major fatty acids (>10 %) were C and summed feature 8 (C 7 and/or C 6). The 16S rRNA gene sequence similarity between strains SYSU D00286 and SYSU D00782 was 100%, and their average nucleotide identity (ANI), average amino acid identity and (AAI) digital DNA-DNA hybridization (dDDH) values were all 100.0 %. Phylogenetic analysis indicated that these two strains belong to the same species of the genus and show the highest sequence similarity to KCTC 72461 (98.2 %) and CCTCC AA 208029 (97.5 %). The ANI, AAI and dDDH values between SYSU D00286 (as well as SYSU D00782) and the other five type strains were all less than or equal to 83.2, 80.1 and 23.6 %, respectively. Based on their phylogenetic, phenotypic and chemotaxonomical features, strains SYSU D00286 and SYSU D00782 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SYSU D00286 (=MCCC 1K04981=CGMCC 1.8626=KCTC 82271).
两株革兰氏阴性菌株,分别命名为 SYSU D00286 和 SYSU D00782,从中国西北部新疆的库姆塔格沙漠的沙样中分离得到。细胞为需氧、非运动、氧化酶和过氧化氢酶均为阳性。生长温度为 4-37°C(最佳温度为 28-30°C),pH 值为 6.0-7.0(最佳 pH 值为 7.0),NaCl 浓度为 0-1.5%(w/v;最佳为 0%)。在 Reasoner 2A 琼脂和营养琼脂上生长,但在 Luria-Bertani 琼脂和胰蛋白酶大豆琼脂上不生长。极性脂类鉴定为双磷脂酰甘油、磷脂酰胆碱、磷脂酰甘油、三种未鉴定的胺脂类、一种未鉴定的糖脂类和两种未鉴定的磷脂类。主要呼吸醌为泛醌-10,主要脂肪酸(>10%)为 C 和总和特征 8(C 7 和/或 C 6)。菌株 SYSU D00286 和 SYSU D00782 的 16S rRNA 基因序列相似度为 100%,其平均核苷酸同一性(ANI)、平均氨基酸同一性(AAI)和(AAI)数字 DNA-DNA 杂交(dDDH)值均为 100.0%。系统发育分析表明,这两株菌属于同一属的同一个种,与 KCTC 72461(98.2%)和 CCTCC AA 208029(97.5%)的序列相似度最高。与其他五个 模式菌株相比,SYSU D00286(以及 SYSU D00782)的 ANI、AAI 和 dDDH 值均小于或等于 83.2、80.1 和 23.6%。基于其系统发育、表型和化学分类学特征,菌株 SYSU D00286 和 SYSU D00782 代表了一个新的 属种,命名为 sp. nov.。模式菌株为 SYSU D00286(=MCCC 1K04981=CGMCC 1.8626=KCTC 82271)。