Biological Sciences Department, California Polytechnic State University, San Luis Obispo, California 93407, United States.
Center for Applications in Biotechnology, California Polytechnic State University, San Luis Obispo, California 93407, United States.
ACS Synth Biol. 2023 Sep 15;12(9):2561-2577. doi: 10.1021/acssynbio.3c00111. Epub 2023 Jul 25.
The fragility of biological systems during storage, transport, and utilization necessitates reliable cold-chain infrastructure and limits the potential of biotechnological applications. In order to unlock the broad applications of existing and emerging biological technologies, we report the development of a novel solid-state storage platform for complex biologics. The resulting solid-state biologics (SSB) platform meets four key requirements: facile rehydration of solid materials, activation of biochemical activity, ability to support complex downstream applications and functionalities, and compatibility for deployment in a variety of reaction formats and environments. As a model system of biochemical complexity, we utilized crudecell extracts that retain active cellular metabolism and support robust levels of transcription and translation. We demonstrate broad versatility and utility of SSB through proof-of-concepts for on-demand biomanufacturing of proteins at a milliliter scale, the activation of downstream CRISPR activity, as well as deployment on paper-based devices. SSBs unlock a breadth of applications in biomanufacturing, discovery, diagnostics, and education in resource-limited environments on Earth and in space.
生物系统在储存、运输和利用过程中的脆弱性需要可靠的冷链基础设施，这也限制了生物技术应用的潜力。为了实现现有和新兴生物技术的广泛应用，我们开发了一种用于复杂生物制剂的新型固态储存平台。由此产生的固态生物制剂（SSB）平台满足四个关键要求：固体材料的简便水合、生物化学活性的激活、支持复杂下游应用和功能的能力，以及与各种反应形式和环境兼容的能力。作为生物化学复杂性的模型系统，我们利用粗提细胞提取物，其保留了活跃的细胞代谢，并支持转录和翻译的强大水平。我们通过按需在毫升规模上进行蛋白质的生物制造、下游 CRISPR 活性的激活，以及在基于纸张的设备上的部署等概念验证，展示了 SSB 的广泛多功能性和实用性。SSB 在地球上和太空中资源有限的环境中，在生物制造、发现、诊断和教育方面解锁了广泛的应用。
