Smith Mark Thomas, Berkheimer Scott D, Werner Christopher J, Bundy Bradley C
Department of Chemical Engineering, Brigham Young University, Provo, Utah.
Biotechniques. 2014 Apr 1;56(4):186-93. doi: 10.2144/000114158. eCollection 2014.
Cell-free protein synthesis (CFPS) is a versatile tool for rapid recombinant protein production and engineering. One drawback of cell-free technology is the necessity to store the major components-cell extracts and energy systems-below freezing in bulky aqueous solutions. Here we describe simple methods for lyophilizing extracts and preparing powdered energy systems for CFPS. These techniques allow for high-density storage of cell-free systems that are more robust against temperature and bacterial degradation. Our methods have the potential to decrease storage expenses, allow for longer shelf-life of cell extracts at room temperature, and enable durable portable protein production technologies.
无细胞蛋白质合成（CFPS）是一种用于快速重组蛋白质生产和工程的通用工具。无细胞技术的一个缺点是需要将主要成分——细胞提取物和能量系统——在冷冻状态下保存在大量水溶液中。在这里，我们描述了冻干提取物和制备用于CFPS的粉末状能量系统的简单方法。这些技术允许对无细胞系统进行高密度存储，使其对温度和细菌降解更具耐受性。我们的方法有可能降低存储成本，使细胞提取物在室温下具有更长的保质期，并实现耐用便携的蛋白质生产技术。
