Li Dapeng, Han Songwei, Zhang Kang, Xu Guowei, Zhang Hong, Chen Fubing, Wang Lei, Liu Qin, Guo Zhiting, Zhang Jingyan, Li Jianxi
Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou, 730050, China.
College of Veterinary Medicine, Hebei Agricultural University, Hebei, 071000, China.
Probiotics Antimicrob Proteins. 2024 Oct;16(5):1638-1656. doi: 10.1007/s12602-023-10124-z. Epub 2023 Jul 26.
Achromobacter marplatensis strain YKS2 isolated from the yak rumen has the feature of producing cellulose. This study aims to analyze the genome and safety of strain YKS2 in vivo, considering its future research and application prospects. The genome of strain YKS2 was sequenced and used for genomic in silico studies. The administration of strain YKS2 in three doses was carried out on mice for 3 days of oral and 7 days of clinical observation tests. The BW, FI, organ indices, gut microbiota, and histological appearances of organs and intestines, along with hematological parameters and serum biochemistry, were measured in mice. The chromosome size of strain YKS2 was 6,588,568 bp, with a GC content of 65.27%. The 6058 coding sequences of strain YKS2 without plasmid were predicted and annotated and have multiple functions. The mice in all groups were alive, with good mental states and functional activities. Compared with the control group, there was no significant difference in the three dose groups on BW, FI, hematological parameters (WBC, LYM, etc.), and serum biochemistry (ALB, ALT, etc.). No abnormalities were observed in the main visceral organs, intestinal tissue, and V/C value in groups. However, the IEL number of duodenum and gut microbiota diversity (Shannon's index) in the high-dose group was significantly higher than in the control group (p < 0.05). Besides, the low dose of strain YKS2 also significantly affected the bacterial abundance of Firmicutes, Actinobacteria, and desulphurizing Bacteroidetes at the phylum level. There was no significant effect at genus levels in groups. In conclusion, the study revealed the genome and potential functional genes of strain YKS2, which is beneficial to understanding the features of the A. marplatensis strain and proved strain YKS2 to be without acute toxicity to mice. However, a long-term feeding toxicity experiment in vivo should be performed to further ensure its potential application value strain in the animal industry.
从牦牛瘤胃中分离出的马普拉滕斯无色杆菌菌株YKS2具有产生纤维素的特性。考虑到其未来的研究和应用前景，本研究旨在分析菌株YKS2的基因组及其在体内的安全性。对菌株YKS2的基因组进行了测序，并用于计算机基因组学研究。以三种剂量对小鼠进行菌株YKS2的给药，进行为期3天的口服和7天的临床观察试验。测量了小鼠的体重、采食量、器官指数、肠道微生物群、器官和肠道的组织学外观，以及血液学参数和血清生化指标。菌株YKS2的染色体大小为6,588,568 bp，GC含量为65.27%。预测并注释了无质粒的菌株YKS2的6058个编码序列，这些序列具有多种功能。所有组的小鼠均存活，精神状态和功能活动良好。与对照组相比，三个剂量组在体重、采食量、血液学参数（白细胞、淋巴细胞等）和血清生化指标（白蛋白、谷丙转氨酶等）方面均无显著差异。各组主要内脏器官、肠道组织和绒毛/隐窝比值均未观察到异常。然而，高剂量组十二指肠的肠上皮内淋巴细胞数量和肠道微生物群多样性（香农指数）显著高于对照组（p < 0.05）。此外，低剂量的菌株YKS2也显著影响了厚壁菌门、放线菌门和脱硫拟杆菌门水平的细菌丰度。各组在属水平上没有显著影响。总之，该研究揭示了菌株YKS2的基因组和潜在功能基因，这有助于了解马普拉滕斯无色杆菌菌株的特性，并证明菌株YKS2对小鼠无急性毒性。然而，应进行长期的体内喂养毒性实验，以进一步确保其在动物产业中的潜在应用价值。
