Characterization of Novel Lytic Bacteriophages of Isolated from a Pneumonia Patient.

spp. are becoming increasingly associated with lung infections in patients suffering from cystic fibrosis (CF). , which is closely related to , has been isolated from the lungs of CF patients and other human infections. This article describes the isolation, morphology and characterization of two lytic bacteriophages specific for an strain isolated from a pneumonia patient. This host strain was the causal agent of hospital acquired pneumonia-the first clinical report of such an occurrence. Full genome sequencing revealed bacteriophage genomes ranging in size from 45901 to 46,328 bp. Transmission electron microscopy revealed that the two bacteriophages AMA1 and AMA2 belonged to the family. Host range analysis showed that their host range did not extend to . The possibility exists for future testing of such bacteriophages in the control of infections such as those seen in CF and other infections of the lungs. The incidence of antibiotic resistance in this genus highlights the importance of seeking adjuncts and alternatives in CF and other lung infections.