Department of Physiology, Anatomy and Microbiology, La Trobe University, Bundoora, Victoria 3083, Australia.
Department of Microbiology, Royal Melbourne Hospital, Victoria 3000, Australia.
Viruses. 2020 Oct 8;12(10):1138. doi: 10.3390/v12101138.
spp. are becoming increasingly associated with lung infections in patients suffering from cystic fibrosis (CF). , which is closely related to , has been isolated from the lungs of CF patients and other human infections. This article describes the isolation, morphology and characterization of two lytic bacteriophages specific for an strain isolated from a pneumonia patient. This host strain was the causal agent of hospital acquired pneumonia-the first clinical report of such an occurrence. Full genome sequencing revealed bacteriophage genomes ranging in size from 45901 to 46,328 bp. Transmission electron microscopy revealed that the two bacteriophages AMA1 and AMA2 belonged to the family. Host range analysis showed that their host range did not extend to . The possibility exists for future testing of such bacteriophages in the control of infections such as those seen in CF and other infections of the lungs. The incidence of antibiotic resistance in this genus highlights the importance of seeking adjuncts and alternatives in CF and other lung infections.
种越来越多地与囊性纤维化（CF）患者的肺部感染相关。与密切相关，已从 CF 患者和其他人类感染的肺部中分离出来。本文描述了从肺炎患者中分离的一株特定溶菌噬菌体的分离、形态和特征。该宿主菌株是医院获得性肺炎的病原体-这是首例此类发生的临床报告。全基因组测序显示噬菌体基因组大小从 45901 到 46328bp 不等。透射电子显微镜显示，两种噬菌体 AMA1 和 AMA2 属于科。宿主范围分析表明，它们的宿主范围不扩展到。未来可以在 CF 和其他肺部感染等感染中测试这些噬菌体，以控制感染。该属中抗生素耐药性的发生率突出了在 CF 和其他肺部感染中寻找辅助剂和替代品的重要性。
