Halas E S, Hunt C D, Eberhardt M J
Physiol Behav. 1986;37(3):451-8. doi: 10.1016/0031-9384(86)90205-2.
Three experiments were conducted to test the behavior of the offspring of rat dams (ZD) fed a mildly zinc deficient diet (10.0 micrograms Zn/g) during pregnancy and lactation. Since zinc deficiency causes anorexia, a second group of rat dams (PF) was fed the same quantity of the diet during gestation and lactation as was consumed by their ZD mates. A third group of rats (AL) was fed the diet ad lib during gestation and lactation. The PF and AL dams were given zinc supplemented (25.0 micrograms Zn/ml) drinking water whereas the ZD dams were given double distilled, deionized drinking water. After weaning, the offspring of all dams were fed Purina Laboratory Chow ad lib until they were 100 days old. The offspring were then reduced to 85% of their ad lib weight and tested on a 17-arm radial maze for memory and learning. In Experiments 1 and 2, the ZD males suffered a significant learning deficit when compared to the AL males. Whereas the PF males suffered a significant learning impairment in Experiment 1, the learning deficit of the PF group was not as severe as the deficit of the ZD group. There was no impairment in reference (long-term) memory for any of the groups. In Experiment 3, significant differences in working (short-term) memory were found among the three groups of females. The ZD group was significantly inferior in working memory when compared to the PF and AL groups. No significant differences in working memory were found between the PF and AL groups.
进行了三项实验，以测试孕期和哺乳期喂食轻度缺锌饮食（10.0微克锌/克）的大鼠母鼠（ZD）所产后代的行为。由于缺锌会导致厌食，第二组大鼠母鼠（PF）在妊娠和哺乳期所喂食的饮食量与它们的ZD配偶所消耗的相同。第三组大鼠（AL）在妊娠和哺乳期自由采食该饮食。PF和AL母鼠饮用补充了锌（25.0微克锌/毫升）的水，而ZD母鼠饮用双蒸去离子水。断奶后，所有母鼠的后代自由采食普瑞纳实验饲料，直至100日龄。然后将后代体重减至自由采食时体重的85%，并在一个17臂放射状迷宫中测试其记忆和学习能力。在实验1和2中，与AL雄性相比，ZD雄性存在显著的学习缺陷。虽然PF雄性在实验1中存在显著的学习障碍，但PF组的学习缺陷不如ZD组严重。任何一组的参考（长期）记忆均未受损。在实验3中，三组雌性大鼠在工作（短期）记忆方面存在显著差异。与PF组和AL组相比，ZD组在工作记忆方面明显较差。PF组和AL组在工作记忆方面未发现显著差异。
