Universidade Federal do Delta do Parnaíba, Núcleo de Pesquisa e Pos-graduação, Parnaíba, PI 64202-020, Brazil.
Universidade Federal do Piauí, Departamento de Morfofisiologia Veterinária, Teresina, PI 64049-550, Brazil.
J Appl Microbiol. 2023 Aug 1;134(8). doi: 10.1093/jambio/lxad162.
The aim of this study was to investigate the antibacterial and antibiofilm potential of cordiaquinones B, E, L, N, and O against different Staphylococci strains, in addition to analyzing in silico the observed effect.
The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined according to CLSI guidelines. The inhibition of biofilm formation was investigated at sub-MICs. Atomic force microscopy (AFM) and density functional theory method were performed. The tested strains of Staphylococcus spp. were susceptible to cordiaquinones B, E, and L, among which cordiaquinone B exerted a bactericidal effect, confirmed by a bacterial growth curve study, against Staphylococcus saprophyticus. Cordiaquinones B and E showed lowest MBC values against S. saprophyticus. AFM revealed that cordiaquinone L reduced the mean cell size of S. saprophyticus. Cordiaquinones B and E inhibited the biofilm formation ability of S. aureus by ∼90%. The in silico analysis suggested that the antimicrobial activity of cordiaquinones is driven by their electron donation capability.
Cordiaquinones inhibit the growth and biofilm formation (virulence factor) of both methicillin-sensitive and methicillin-resistant Staphylococci strains, indicating their antimicrobial potential.
本研究旨在探讨 Cordiaquinones B、E、L、N 和 O 对不同葡萄球菌菌株的抗菌和抗生物膜潜力，并分析其观察到的效果的计算分析。
根据 CLSI 指南测定最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。在亚 MIC 下研究了生物膜形成的抑制作用。进行了原子力显微镜（AFM）和密度泛函理论方法的研究。测试的葡萄球菌菌株对 Cordiaquinones B、E 和 L 敏感，其中 Cordiaquinone B 通过细菌生长曲线研究证实对腐生葡萄球菌具有杀菌作用。Cordiaquinone B 和 E 对腐生葡萄球菌的最低 MBC 值。AFM 显示 Cordiaquinone L 降低了腐生葡萄球菌的平均细胞大小。Cordiaquinones B 和 E 通过 ∼90%抑制金黄色葡萄球菌的生物膜形成能力。计算分析表明，Cordiaquinones 的抗菌活性是由其电子供体能力驱动的。
Cordiaquinones 抑制了耐甲氧西林和耐甲氧西林金黄色葡萄球菌菌株的生长和生物膜形成（毒力因子），表明其具有抗菌潜力。
