Institut de Biologia Evolutiva (CSIC-Universitat Pompeu Fabra), Barcelona, Spain.
Agricultural Entomology, Insect Physiology, Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
Insect Mol Biol. 2023 Dec;32(6):689-702. doi: 10.1111/imb.12866. Epub 2023 Jul 27.
The transcription factor Ftz-f1 has multiple functions in insect development in a spatial-temporal line. One of these roles is in the insect ovaries, specifically in the regulation of steroidogenic enzymes production. We studied the function of F in Blattella germanica oogenesis, as it shows two moments of high expression in ovaries: before the imaginal moult, and just before ovulation in the adult. Injecting dsftz-f1 into adult females, either just after the imaginal moult or just prior to choriogenesis, prevented oviposition, with differences between the two approaches. In 3-day-old adult females treated with dsftz-f1 just after the emergence, the expression of ftz-f1 was not modified, but the steroidogenic genes increased their expression. ftz-f1 transcript levels in the ovaries of 5-day-old dsftz-f1-treated females were significantly depleted, and the expression levels of the same steroidogenic genes began to decrease. These results suggest that Ftz-f1 regulates the expression of steroidogenic genes in B. germanica, with phm possibly being a key target. Ftz-f1 has a different temporal function in the cytoskeleton of follicular cells of the basal ovarian follicles. Early in the gonadotrophic cycle, Ftz-f1 promotes the expression of genes related to the cytoskeleton and muscle proteins, while at the end of the cycle it maintains the expression levels of these genes, thus ensuring correct ovulation.
转录因子 Ftz-f1 在昆虫发育的时空线上具有多种功能。其中一个作用是在昆虫卵巢中，特别是在调节类固醇生成酶的产生方面。我们研究了 F 在德国蟑螂卵发生中的功能，因为它在卵巢中表现出两个高表达的时刻：在羽化前和成虫排卵前。在羽化后或在卵黄发生前将 dsftz-f1 注射到成年雌性体内，会阻止产卵，两种方法之间存在差异。在刚羽化后用 dsftz-f1 处理的 3 天龄成年雌性中，ftz-f1 的表达没有改变，但类固醇生成基因的表达增加。在 5 天龄的 dsftz-f1 处理的雌性的卵巢中，ftz-f1 的转录本水平显著减少，并且相同的类固醇生成基因的表达水平开始下降。这些结果表明，Ftz-f1 调节 B. germanica 中类固醇生成基因的表达，phm 可能是一个关键靶标。Ftz-f1 在基底卵巢滤泡的滤泡细胞骨架中有不同的时间功能。在性腺激素周期的早期，Ftz-f1 促进与细胞骨架和肌肉蛋白相关的基因的表达，而在周期结束时，它维持这些基因的表达水平，从而确保正确的排卵。
